Nankai University 5 articles published in JoVE Cancer Research Analysis of Side Population in Solid Tumor Cell Lines Xiaoli Dong1, Yingying Wei1, Tao Xu1, Xiaoyue Tan1,2,3, Na Li1,2,3 1School of Medicine, Nankai University, 2Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, 3Collaborative Innovation Center for Biotherapy, Nankai University A convenient, fast, and cost-effective method to measure the proportion of side population cells in solid tumor cell lines is presented. Medicine Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis Kaiyue Zhang1, Chen Wang1, Ran Wang2, Shang Chen1, Zongjin Li1 1Nankai University School of Medicine, 2State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging. Behavior Behavioral Assessments of Spontaneous Locomotion in a Murine MPTP-induced Parkinson's Disease Model Pei-En Jiang1, Qiu-Han Lang1, Qin-Yi Yu1, Xin-Yu Tang1, Qian-Qian Liu1, Xin-Yu Li1, Xi-Zeng Feng1 1State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University We describe establishment of a murine model for Parkinson's disease using MPTP, and behavioral assessments using cylinder and open field tests to measure motor function. We then use L-DOPA as one example to show how to apply this model in the study of PD drugs. Biology Implantation of Electrospun Vascular Grafts with Optimized Structure in a Rat Model Kang Qin1, Yifan Wu1, Yiwa Pan1, Kai Wang1, Deling Kong1, Qiang Zhao1 1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials (Ministry of Education), College of Life Sciences, Nankai University Here, we present a modified electrospinning method to fabricate PCL vascular grafts with thick fibers and large pores, and describe a protocol to evaluate the in vivo performance in a rat model of abdominal aorta replacement. Immunology and Infection In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging Yucheng Wang1,2,3, Olivia D. Harrington1, Ying Wang1, Clinton K. Murray4, Michael R. Hamblin1, Tianhong Dai1 1Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, 2Department of Medical Oncology, Beijing Institute of Translational Medicine, Chinese Academy of Sciences, 3Cancer Center, Aviation General Hospital, Beijing, 4Infectious Disease Service, Brooke Army Medical Center Infections caused by multidrug-resistant (MDR) bacterial strains have emerged as a serious threat to public health, necessitating the development of alternative therapeutics. We present a protocol to evaluate the effectiveness of antimicrobial blue light (aBL) therapy for MDR Acinetobacter baumannii infections in mouse burns by using bioluminescence imaging.
