1Faculty of Life Sciences and the Institute for Nanotechnology & Advanced Materials, Bar-Ilan University
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here, we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.
Published July 8, 2013. Keywords: Bioengineering, Genetics, Biomedical Engineering, Molecular Biology, Medicine, Genomics, Nanotechnology, Nanomedicine, DNA origami, nanorobot, caDNAno, DNA, DNA Origami, nucleic acids, DNA structures, CAD, sequencing
JoVE Immunology and Infection
1Department of Physics, Bar-Ilan University, 2The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 3Department of Chemistry, Bar-Ilan University, 4Faculty of Engineering, Bar-Ilan University
We show that a developed biomedical device involving continuous or pulsed visible laser based treatment that is combined with antibiotic treatment (gentamycin), results in a statistically significant synergistic effect leading to a reduction in the viability of P. aeruginosa PAO1, by 8 log's compared to antibiotic treatment alone.
Published July 2, 2013. Keywords: Microbiology, Infection, Infectious Diseases, Cellular Biology, Molecular Biology, Biophysics, Chemistry, Biomedical Engineering, Bacteria, Photodynamic therapy, Medical optics, Bacterial viability, Antimicrobial treatment, Laser, Gentamycin, antibiotics, reactive oxygen species, pathogens, microorganisms, cell culture
1The Mina & Everard Goodman Faculty of Life Sciences, The Nanotechnology Institute, Bar-Ilan University
We present a microfluidic approach for the expression of protein arrays. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for experimental use.
Published August 23, 2012. Keywords: Bioengineering, Genetics, Chemistry, Molecular Biology, In vitro protein expression, microfluidics, protein microarray, systems biology, high-throughput, screening
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University
RNA polymerase II transcriptional kinetics are measured on specific genes in living cells. mRNAs transcribed from the gene of interest are fluorescently tagged and using Fluorescence Recovery After Photobleaching (FRAP) the in vivo kinetics of transcriptional elongation are obtained.
Published August 25, 2011. Keywords: Cell Biology, mRNA transcription, nucleus, live-cell imaging, cellular dynamics, FRAP