University of Science and Technology (Korea) View Institution's Website 11 articles published in JoVE Biology Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms Joo-Sung Kim1,2, Jihoon Lim3 1Korea Food Research Institute, 2Department of Food Biotechnology, Korea University of Science and Technology, 3Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter. Cancer Research Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells Jina Lee*1,2, Joo-Eun Lee*3, Seil Kim1,4,5, Dukjin Kang1, Hee Min Yoo1 1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST) Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways. Medicine Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans Tram Anh Ngoc Le1, Baskar Selvaraj1,2, Jae Wook Lee1,2, Kyungsu Kang1,2 1Gangneung Institute of Natural Products, Korea Institute of Science and Technology, 2Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST) This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases. Engineering Fabrication of Robust Nanoscale Contact between a Silver Nanowire Electrode and CdS Buffer Layer in Cu(In,Ga)Se2 Thin-film Solar Cells Sangyeob Lee1,2, Kyung Soo Cho2, Soomin Song3, Kihwan Kim3,4, Young-Joo Eo3,4, Jae Ho Yun3,4, Jihye Gwak3,4, Choong-Heui Chung1,2 1Department of Materials Science and Engineering, Hanbat National University, 2Department of Materials and Manufacturing Engineering, Hanbat National University, 3Photovoltaics Laboratory, Korea Institute of Energy Research, 4Renewable Energy Engineering, University of Science and Technology, Daejeon In this protocol, we describe the detailed experimental procedure for the fabrication of a robust nanoscale contact between a silver nanowire network and CdS buffer layer in a CIGS thin-film solar cell. Engineering An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter Ha Eun Shim*1,2, Sajid Mushtaq*1,3, Jongho Jeon1,3 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Chemistry, Kyungpook National University, 3Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology An efficient method for the rapid and ion-selective desalination of radioactive iodine in several aqueous solutions is described by using gold nanoparticles-immobilized cellulose acetate membrane filters. Neuroscience Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev1,2, Jinho Chung1, Dajung Jung1,3, Farnaz Sharif1,2, Ju-Young Lee1,2, Soyoun Kim1, Sebastien Royer1,2 1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus. Biochemistry Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells Md Nazmul Huda1,2, Erdenedolgor Erdene-Ochir1,2, Cheol-Ho Pan1,2 1Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding. Chemistry An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group Jongho Jeon1,2, Ha Eun Shim1, Sajid Mushtaq1,2, Mi Hee Choi1, Sang Hyun Park1,2, Dae Seong Choi1, Beom-Su Jang1,2 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described. Biochemistry Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer Haseong Kim*1, Gui Hwan Han*1, Yaoyao Fu1, Jongsik Gam2, Seung Goo Lee1,3 1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies, Konyang University, 3Biosystems and Bioengineering Program, University of Science and Technology This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content. Neuroscience Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits Hye Yun Kim1,2, Dongkeun K. Lee1, Bo-Ryehn Chung1,3, Hyunjin V. Kim1,4, YoungSoo Kim1,4 1Center for Neuro-Medicine, Korea Institute of Science and Technology, 2Research Institute, GoshenBiotech, Inc., 3Department of Chemical and Biological Engineering, Princeton University, 4Biological Chemistry Program, Korea University of Science and Technology The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice. Neuroscience High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Mina Lee1,2, Dongwook Kim1,2, Hee-Sup Shin1,2, Ho-Geun Sung3, Jee Hyun Choi1,2 1Center for Neural Science , Korea Institute of Science and Technology (KIST), 2Department of Neuroscience, University of Science and Technology, 3Fab Service Department, Korea Advanced Nano Fab Center In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.
