Baker IDI Heart and Diabetes Institute View Institution's Website 4 articles published in JoVE Bioengineering Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA Tatjana Michel1, Antonia Link1, Meike-Kristin Abraham1,2, Christian Schlensak1, Karlheinz Peter2,3, Hans-Peter Wendel1, Xiaowei Wang2,3, Stefanie Krajewski1 1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, 2Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, 3Department of Medicine, Monash University Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA. Medicine Body Composition and Metabolic Caging Analysis in High Fat Fed Mice Graeme I. Lancaster1, Darren C. Henstridge1 1Baker Heart and Diabetes Institute This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques. Medicine Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel Thomas Bonnard1, Christoph E. Hagemeyer1 1Vascular Biotechnology Laboratory, Baker IDI Heart and Diabetes Institute The FeCl3 induced thrombosis model in mice is described herein. A method to monitor thrombus growth by intravital microscopy observation on a mesenteric vessel and by blood flow measurement in the carotid artery is presented. Medicine Cholesterol Efflux Assay Hann Low1, Anh Hoang1, Dmitri Sviridov1 1Baker IDI Heart and Diabetes Institute The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.
