Braunschweig University of Technology View Institution's Website 5 articles published in JoVE Engineering Scattering And Absorption of Light in Planetary Regoliths Karri Muinonen1,2, Timo Väisänen1, Julia Martikainen1, Johannes Markkanen1,3, Antti Penttilä1, Maria Gritsevich1,4, Jouni Peltoniemi1,2, Jürgen Blum5, Joonas Herranen1, Gorden Videen6,7, Göran Maconi1, Petteri Helander1, Ari Salmi1, Ivan Kassamakov1, Edward Haeggström1 1Department of Physics, University of Helsinki, 2Finnish Geospatial Research Institute FGI, National Land Survey, 3Max Planck Institute for Solar System Research, 4Institute of Physics and Technology, Ural Federal University, 5Institut für Geophysik und Extraterrestrische Physik, Technische Universität Braunschweig, 6Space Science Institute, 7Army Research Laboratory Numerical and experimental methods are presented for multiple scattering of light in discrete random media of densely-packed particles. The methods are utilized to interpret the observations of asteroid (4) Vesta and comet 67P/Churyumov-Gerasimenko. Environment Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector Michaela Hoenigsberger1, Alexey G. Kopchinskiy2, Alexandra Parich1, Karsten Hiller3,4, Alice Laciny5, Herbert Zettel5, Linda B.L. Lim6, Kamariah A. Salim7, Irina S. Druzhinina2, Rainer Schuhmacher1 1Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Austria, 2Institute of Chemical, Environmental and Biological Engineering, TU Vienna, Austria, 3Department of Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Germany, 4Institute for Biochemistry, Biotechnology and Bioinformatics, University of Technology (TU) Braunschweig, Germany, 52nd Zoological Department, Natural History Museum Vienna, Austria, 6Chemical Sciences, Universiti Brunei Darussalam, Brunei, 7Environmental and Life Sciences, Universiti Brunei Darussalam, Brunei Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described. Biochemistry Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis Markus Nachbar1, Jorrit Maul1, Matthias Stein1, Hermann Wätzig1 1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers. Developmental Biology Culture and Transfection of Zebrafish Primary Cells Giulio Russo1,2, Franziska Lehne1, Sol M. Pose Méndez1, Stefan Dübel2, Reinhard W. Köster1, Wiebke A. Sassen1 1Division of Cellular and Molecular Neurobiology, Zoological Institute, Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig University of Technology We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain. Neuroscience Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines Kenric J. Hoegler1, Martin Distel2, Reinhard W. Köster3, John H. Horne1 1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.