German Sport University Cologne View Institution's Website 2 articles published in JoVE Biochemistry Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins Marijke Grau1, Lennart Kuck2 1Department of Molecular and Cellular Sports Medicine, German Sport University Cologne, 2Biorheology Research Laboratory, Menzies Health Institute, Griffith University Gold Coast Capturing dynamic changes in the protein activation of enucleated red blood cells poses methodological challenges, like the preservation of dynamic changes to acute stimuli for later assessment. The presented protocol describes sample preparation and staining techniques that enable preservation and analysis of relevant protein changes and subsequent detection. Neuroscience Coherence between Brain Cortical Function and Neurocognitive Performance during Changed Gravity Conditions Vera Brümmer1, Stefan Schneider1, Tobias Vogt1, Heiko Strüder1, Heather Carnahan2, Christopher D. Askew3, Roland Csuhaj4 1Institute of Movement and Neurosciences, German Sport University Cologne, 2Deptartment of Surgical Skills, University of Toronto, 3School of Human Movement Studies, Institute of Health and Biomedical Innovation, Queensland University of Technology, 4Brain Products GmbH, Scientific Support, Gilching, Germany The effect of weightlessness and hypergravity on both hemodynamic and electrophysiological processes in the brain is going to be followed during parabolic flight by EEG and NIRS techniques. A feasibility study of a more complex experiment, which is planned to carry out during medium- and long-term space flight.