Leibniz-Institut für Molekulare Pharmakologie View Institution's Website 1 article published in JoVE Engineering Hyperpolarized Xenon for NMR and MRI Applications Christopher Witte1, Martin Kunth1, Jörg Döpfert1, Federica Rossella1, Leif Schröder1 1ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmakologie The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are given.