Universite Paris Diderot View Institution's Website 6 articles published in JoVE Medicine Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis Mathieu Manassero1,2, Adeline Decambron1,2, Bui Truong Huu Thong1, Véronique Viateau1,2, Morad Bensidhoum1, Hervé Petite1 1Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires (B2OA - UMR CNRS 7052), Université Paris Diderot, 2Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity. Medicine Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models Jeremie Cosette1,2,3, Rym Ben Abdelwahed2, Sabrina Donnou-Triffault1,4,5, Catherine Sautès-Fridman2, Patrice Flaud*3, Sylvain Fisson*1,4,5 1Genethon, 2Cordeliers Research Center, INSERM U872, 3Matter and Complex Systems Laboratory, Université Paris Diderot, 4INSERM U951, 5UMR_S951, Université d'Evry Val d'Essonne Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models. Neuroscience Purification of Mouse Brain Vessels Anne-Cécile Boulay1,2,3, Bruno Saubaméa4, Xavier Declèves4, Martine Cohen-Salmon1,2,3 1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes. Chemistry High Precision Zinc Isotopic Measurements Applied to Mouse Organs Frédéric Moynier1, Marie Le Borgne2 1Institut de Physique du Globe de Paris, Institut Universitaire de France, Université Paris Diderot, 2INSERM (Institut National de la Santé et de la Recherche Médicale), U1148: Laboratory for Vascular Translational Science, Université Paris Diderot We present the technique to measure with high precision zinc isotope ratios in mouse organs. Biology AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions Alexis Peaucelle1,2 1Laboratoire Matière et Systèmes Complexes, UFR de Physique, Université Paris Diderot, 2Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis. Neuroscience Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices Ulrike Pannasch*1, Jérémie Sibille*1,2, Nathalie Rouach1 1Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, 2Paris Diderot University The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.