Trinity College Dublin View Institution's Website 10 articles published in JoVE Environment Sampling, Identification and Characterization of Microplastics Release from Polypropylene Baby Feeding Bottle during Daily Use Dunzhu Li*1,2, Luming Yang*1,2, Rachel Kavanagh1, Liwen Xiao2,3, Yunhong Shi1,2, Daniel K. Kehoe1, Emmet D. Sheerin1,4, Yurii K. Gun’ko4,5, John J. Boland1,4, Jing Jing Wang1 1AMBER Research Centre and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, 2Department of Civil, Structural and Environmental Engineering, Trinity College Dublin, 3TrinityHaus, Trinity College Dublin, 4School of Chemistry, Trinity College Dublin, 5BEACON, Bioeconomy SFI Research Centre, University College Dublin This study detailed a reliable and cost-effective protocol for microplastics collection and detection from the daily use of plastic products. Cancer Research Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds Ciara Gallagher*1,2,3, Catherine Murphy*1,2,3, Graeme Kelly4, Fergal J. O’Brien3,5,6, Olga Piskareva1,2,3,6,7 1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland This paper lists the steps required to seed neuroblastoma cell lines on previously described three-dimensional collagen-based scaffolds, maintain cell growth for a predetermined timeframe, and retrieve scaffolds for several cell growth and cell behavior analyses and downstream applications, adaptable to satisfy a range of experimental aims. Developmental Biology Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells Balta Lucendo-Villarin1, Hassan Rashidi1,2, Sharmin Alhaque1,3, Lena Fischer1,4, Jose Meseguer-Ripolles1, Yu Wang1, Cliona O'Farrelly4, Michael Themis3, David C. Hay1 1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application. Neuroscience Measurement & Analysis of the Temporal Discrimination Threshold Applied to Cervical Dystonia Rebecca B Beck*1, Eavan M McGovern*1,2,3, John S Butler4, Dorina Birsanu1, Brendan Quinlivan1, Ines Beiser1,2,3, Shruti Narasimham1, Sean O'Riordan2,3, Michael Hutchinson2,3, Richard B Reilly1,5 1School of Engineering, Trinity College Dublin, The University of Dublin, 2 Methods for the measurement and analysis of the temporal discrimination threshold are presented, and its application to the study of the pathogenesis of cervical dystonia are discussed. Engineering Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol Claudia Backes1, Damien Hanlon2, Beata M. Szydlowska2, Andrew Harvey2, Ronan J. Smith2, Thomas M. Higgins1, Jonathan N. Coleman2 1Chair of Applied Physical Chemistry, Ruprecht-Karls University Heidelberg, 2School of Physics and CRANN, Trinity College Dublin A protocol for the liquid exfoliation of layered materials to nanosheets, their size selection and size measurement by microscopic and spectroscopic techniques is presented. Medicine Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections Natalia Muñoz-Wolf1,2, Analía Rial1, José M. Saavedra1, José A. Chabalgoity1 1Departamento de Desarrollo Biotecnológico, Universidad de la República, 2Present Affiliation: Trinity Biomedical Science Institute, Trinity College Dublin The present work illustrates the convenience of using sublingual immunotherapy to boost the innate immune response in the lungs and confer protection against acute pneumococcal pneumonia in mouse. Bioengineering Harmonic Nanoparticles for Regenerative Research Flavio Ronzoni1, Thibaud Magouroux2, Remi Vernet1, Jérôme Extermann3, Darragh Crotty4, Adriele Prina-Mello5, Daniel Ciepielewski6, Yuri Volkov4, Luigi Bonacina2, Jean-Pierre Wolf2, Marisa Jaconi1 1Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 2Physics Department, GAP-Biophotonics, University of Geneva, 3Laboratoire d'Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l'Ingénieur, École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine, Trinity College Dublin, 5School of Medicine and CRANN, Trinity College Dublin, 6Nikon AG Instruments Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented. Biology Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography Dianfan Li1, Coilín Boland1, David Aragao1, Kilian Walsh1, Martin Caffrey1 1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography. Biology Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases Dianfan Li1, Coilín Boland1, Kilian Walsh1, Martin Caffrey1 1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced. Biology Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases Martin Caffrey1, Christopher Porter1 1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine, Trinity College Dublin Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.