University of Naples View Institution's Website 2 articles published in JoVE Immunology and Infection Bone Marrow-derived Macrophage Production Virginie Trouplin1, Nicolas Boucherit1, Laurent Gorvel1, Filippo Conti1, Giovanna Mottola1,2, Eric Ghigo1 1CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université, 2Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II" Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow. Biology Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc Rosario Vicidomini1, Giuseppe Tortoriello1, Maria Furia1, Gianluca Polese1 1Dipartimento di Biologia Strutturale e Funzionale, University of Naples Laser microdissection was applied to analyse gene expression profiling in specific compartments of Drosophila wing disc subjected to localised RNAi in vivo. RNA extracted from equivalent areas of silenced and unsilenced compartments was analysed by quantitative RT-PCR to determine comparative gene expression profiling within the context of native tissue microecology.
