12 articles published in JoVE
13D Machine Vision Laboratory, Department of Mechanical Engineering, Iowa State University
This video describes the fundamentals of digital fringe projection techniques, which provide dense 3D measurements of dynamically changing surfaces. It also demonstrates the design and operation of a high-speed binary defocusing system based on these techniques.
Published December 3, 2013. Keywords: Physics, Structured light, Fringe projection, 3D imaging, 3D scanning, 3D video, binary defocusing, phase-shifting
1School of Environmental and Forest Sciences, University of Washington, 2Center for Agricultural and Rural Development, Department of Economics, Iowa State University, 3Department of Civil, Architectural, and Environmental Engineering, North Carolina A&T University, 4Iowa Geological and Water Survey
This work demonstrates an integration of a water quality model with an optimization component utilizing evolutionary algorithms to solve for optimal (lowest-cost) placement of agricultural conservation practices for a specified set of water quality improvement objectives. The solutions are generated using a multi-objective approach, allowing for explicit quantification of tradeoffs.
Published December 9, 2012. Keywords: Environmental Sciences, Plant Biology, Civil Engineering, Forest Sciences, Water quality, multiobjective optimization, evolutionary algorithms, cost efficiency, agriculture, development
1Department of Chemical and Biological Engineering, Iowa State University
This method describes the combinatorial synthesis of biodegradable polyanhydride film and nanoparticle libraries and the high-throughput detection of protein release from these libraries.
Published September 6, 2012. Keywords: Bioengineering, combinatorial, high-throughput, polymer synthesis, polyanhydrides, nanoparticle fabrication, release kinetics, protein delivery
1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Chemistry, Iowa State University
In this article, a high throughput method is presented for the synthesis of oligosaccharides and their attachment to the surface of polyanhydride nanoparticles for further use in targeting specific receptors on antigen presenting cells.
Published July 6, 2012. Keywords: Bioengineering, Chemical Engineering, High-throughput, Automation, Carbohydrates, Synthesis, Polyanhydrides, Nanoparticles, Functionalization, Targeting, Fluorous Solid Phase Extraction
1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University
In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.
Published June 8, 2012. Keywords: Bioengineering, Microbiology, ImageStream, phagocytosis, nanoparticles, pathogen, bacteria, Salmonella, imaging, multi-spectral imaging, flow cytometry
1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University
Herein, we describe protocols for harvesting murine alveolar macrophages, which are resident innate immune cells in the lung, and examining their activation in response to co-culture with polyanhydride nanoparticles.
Published June 8, 2012. Keywords: Bioengineering, Microbiology, alveolar macrophages, AMɸ, lung lavage, polyanhydride, nanoparticles, harvesting, activation
1Department of Genetics, Development and Cell Biology, Neuroscience Program, Iowa State University
A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.
Published April 19, 2012. Keywords: Neuroscience, Single-cells, transcriptomics, gene expression, cell-type markers, retina, neurons, genetics
1Animal Science Department, Iowa State University
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.
Published August 5, 2011. Keywords: Developmental Biology, embryo, cryopreservation, cattle, sheep, goats
JoVE Immunology and Infection
1Center for Meat Safety and Quality, Department of Animal Sciences, Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
Published October 18, 2010. Keywords: Immunology, Salmonella, adhesive tape, rapid detection, fresh produce, fluorescence in situ hybridization, fluorescence microscopy, flow cytometry
1Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University
This video protocol illustrates the squash technique used in the Johansen laboratory to prepare Drosophila polytene chromosomes for antibody labeling.
Published February 9, 2010. Keywords: Cellular Biology, polytene squash preparations, antibody labeling, chromosomes, Drosophila
1Department of Entomology, Iowa State University
In this video, we demonstrate oral infection techniques of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.
Published September 3, 2008. Keywords: Plant Biology, Springer Protocols, Baculovirus insecticides, recombinant baculovirus, insect pest management
1Department of Entomology, Iowa State University
In this video, we demonstrate two microapplicator techniques used to infect of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.
Published August 23, 2008. Keywords: Plant Biology, Springer Protocols, Baculovirus insecticides, recombinant baculovirus, insect pest management