15 articles published in JoVE
1Fischell Department of Bioengineering, University of Maryland, College Park
Lymph nodes are the immunological tissues that orchestrate immune response and are a critical target for vaccines. Biomaterials have been employed to better target lymph nodes and to control delivery of antigens or adjuvants. This paper describes a technique combining these ideas to inject biocompatible polymer particles into lymph nodes.
Published January 2, 2014. Keywords: Bioengineering, biomaterial, immunology, microparticle, nanoparticle, vaccine, adjuvant, lymph node, targeting, polymer
1Fischell Department of Bioengineering, University of Maryland, 2Institute for Bioscience and Biotechnology Research, University of Maryland
Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.
Published October 17, 2013. Keywords: Bioengineering, Antigens, Enzymes, Biological Therapy, bioengineering (general), Pharmaceutical Preparations, Macromolecular Substances, Therapeutics, Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, targeted nanocarriers, transcellular transport, epithelial cells, tight junctions, transepithelial electrical resistance, endocytosis, transcytosis, radioisotope tracing, immunostaining
1Department of Cell Biology and Molecular Genetics, University of Maryland
Live imaging of cells exposed to the lipophilic dye FM1-43 allows precise determination of the kinetics by which pore-forming toxins are removed from the plasma membrane. This is a sensitive assay that can be used to assess requirements for Ca2+, sphingomyelinase and other factors on plasma membrane repair.
Published August 25, 2013. Keywords: Cellular Biology, Molecular Biology, Infection, Medicine, Immunology, Biomedical Engineering, Anatomy, Physiology, Biophysics, Genetics, Bacterial Toxins, Microscopy, Video, Endocytosis, Biology, Cell Biology, streptolysin O, plasma membrane repair, ceramide, endocytosis, Ca2+, wounds
JoVE Immunology and Infection
1Department of Microbiology and Immunology, University of Maryland
Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.
Published December 29, 2012. Keywords: Immunology, Medicine, Molecular Biology, Cellular Biology, Microbiology, Cancer Biology, Natural killer T cells, in vitro expansion, cancer immunology, artificial antigen presenting cells, adoptive transfer
JoVE Immunology and Infection
1Institute for Bioscience and Biotechnology Research, University of Maryland
A novel directed evolution method specific to the field of thermostability engineering was developed and consequently validated for bacteriolytic enzymes. After only one round of random mutagenesis, an evolved bacteriolytic enzyme, PlyC 29C3, displayed greater than twice the residual activity when compared to the wild-type protein after elevated temperature incubation.
Published November 7, 2012. Keywords: Immunology, Molecular Biology, Genetics, Microbiology, directed evolution, thermal behavior, thermostability, endolysin, enzybiotic, bacteriolytic, antimicrobial, therapeutic, PlyC
1Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, 2Department of Neurology, Johns Hopkins School of Medicine, 3University of Maryland, 4Experimental Neurology, Biogen Idec, 5The Brain Science Institute, Johns Hopkins School of Medicine, 6Department of Pediatrics, Johns Hopkins School of Medicine
This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.
Published June 20, 2012. Keywords: Neuroscience, Physiology, Medicine, periventricular leukomalacia, oligodendrocytes, glial restricted precursors, spinal cord, cell culture
1Fischell Department of Bioengineering, University of Maryland, 2Institute for Bioscience and Biotechnology Research, University of Maryland, 3Department of Materials Science and Engineering, University of Maryland
This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.
Published June 6, 2012. Keywords: Bioengineering, Biomedical Engineering, electrodeposition, biofabrication, chitosan, alginate, lab-on-a-chip, microfluidic, DTRA
1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 4The Program in Neuroscience, University of Maryland
The forced swim test is validated as an experimental approach to assess potential antidepressant efficacy in rodents. Experimental animals are placed in a tank of water and escape-related mobility behavior is quantified. The common procedures for the mouse version of this test are described.
Published January 29, 2012. Keywords: Neuroscience, animal models, behavioral analysis, neuroscience, neurobiology, mood disorder, depression, mood stabilizer, antidepressant, forced swimming test, FST
1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3The Program in Neuroscience, University of Maryland, 4Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
Published January 28, 2012. Keywords: Neuroscience, animal models, behavioral analysis, neuroscience, neurobiology, mood disorder, depression, mood stabilizer, antidepressant
1Department of Cell Biology and Molecular Genetics, University of Maryland, 2Department of Biotechnology and Microbiology, Vilnius University
Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.
Published October 24, 2011. Keywords: Cell Biology, Ribosome, purification, DNA, yeast, chromatography, Saccharomyces cerevisiae
1Department of Neural and Pain Sciences, University of Maryland
A technique is described to quantify the in vivo physiological response of mammalian neurons during movement and correlate the physiology of the neuron with neuronal morphology, neurochemical phenotype and synaptic microcircuitry.
Published April 21, 2011. Keywords: Neuroscience, neurophysiology, sensory neuron, motor control, proprioception, neurotransmitter, sensorimotor integration, rat
JoVE Immunology and Infection
1Department of Veterinary Medicine, University of Maryland, 2Department of Entomology, Connecticut Agricultural Experiment Station
Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
Published February 14, 2011. Keywords: Infection, Lyme disease, tick, microinjection, Borrelia burgdorferi, immunofluorescence microscopy
1Dept. Of Cell Biology and Molecular Genetics, University of Maryland
This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)
Published June 12, 2010. Keywords: Plant Biology, Bimolecular Fluorescence Complementation (BiFC), particle bombardment, protein-protein interaction, onion cells, Helios Gene Gun
1Department of Psychology, Neuroscience and Cognitive Science Program , University of Maryland
Non-invasive measurements of neural activity patterns in freely behaving animals are obtained by combining neurophysiological recordings with high speed videography.
Published July 22, 2009. Keywords: Neuroscience, Electrophysiology, bath electrodes, neurons, behavior
1Genome Technology Branch, National Human Genome Research Institute, 2Neuroscience and Cognitive Science Program, University of Maryland
The inner ear sensory epithelium of adult zebrafish is a good model system for understanding the mechanisms of hair cell regeneration in adult vertebrates. This protocol demonstrates the fine dissection of the epithelia, through which we can get tissue samples for studying the regenerative events at cellular and subcellular levels.
Published May 8, 2009. Keywords: Neuroscience, zebrafish, dissection, inner ear, sensory epithelia, hair cell, regeneration