University of Maryland, Baltimore County View Institution's Website 9 articles published in JoVE Chemistry Curation of Computational Chemical Libraries Demonstrated with Alpha-Amino Acids Christopher Mayer-Bacon1, Mehmet Aziz Yirik2 1Biological Sciences Department, University of Maryland-Baltimore County, 2Institute for Inorganic and Analytical Chemistry, Friedrich-Schiller University The purpose of this protocol is to efficiently generate and curate small-molecule structure libraries using open-source software. Biochemistry Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins Danté Johnson1, Benjamin Punshon-Smith2, Jessica A. Espino1, Anne Gershenson3, Lisa M. Jones1 1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP). Biology Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay Shonda M. Campbell1, Michelle Starz-Gaiano1, Jeff Leips1 1Department of Biology, University of Maryland Baltimore County This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria. Genetics Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae Meagan Jezek1, Alison Jacques1, Deepika Jaiswal1, Erin M. Green1 1Department of Biological Sciences, University of Maryland Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome. Developmental Biology Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo Rebecca J. McFarland*1, Sharlene P. Brown*1, Eudorah Vital1, Jonathan M. Werner1, Rachel M. Brewster1 1Department of Biological Sciences, University of Maryland, Baltimore County Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically. Chemistry Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography Gregory T. Winter1, Joshua A. Wilhide1, William R. LaCourse1 1Department of Chemistry and Biochemistry, University of Maryland, Baltimore County This paper presents a protocol for the visualization of gaseous streams of an ambient ionization source using schlieren photography and mass spectrometry. Developmental Biology Upright Imaging of Drosophila Egg Chambers Lathiena Manning1, Michelle Starz-Gaiano1 1Department of Biological Sciences, University of Maryland Baltimore County The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium. Biology An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization David Dunston1, Sarah Ashby*1, Kurt Krosnowski*1, Tatsuya Ogura1, Weihong Lin1 1Biological Sciences, University of Maryland Baltimore County Neuroscience Labeling and Imaging Cells in the Zebrafish Hindbrain Pradeepa Jayachandran1, Elim Hong2, Rachel Brewster1 1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.
