Maine Medical Center Research Institute View Institution's Website 5 articles published in JoVE Medicine Noninvasive Electrocardiography in the Perinatal Mouse Lindsey A. Fitzsimons1,2, Victoria L. Brewer3, John Forrester2, Adrian M. Moran4, Kerry L. Tucker1,2 1Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, 2Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England. Biddeford, ME, 3College of Arts and Sciences, University of New England, Biddeford, ME, 4Department of Pediatrics, Division of Pediatric Cardiology, Maine Medical Center, Portland, ME Here, we present a noninvasive electrocardiography (ECG) protocol, optimized for early postnatal mice, that does not require the use of anesthetics. Biology Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells S. Spencer Scott1, Xuehui Yang1, Michael Robich1, Lucy Liaw1, Joshua M. Boucher1 1Maine Medical Center Research Institute The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. Developmental Biology Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice David E. Maridas1, Elizabeth Rendina-Ruedy1, Phuong T. Le1, Clifford J. Rosen1 1Maine Medical Center Research Institute In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts. Biology Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix Matthew K. Stephenson1, Sean Lenihan2,3, Roman Covarrubias2,3, Ryan M. Huttinger2,3, Richard J. Gumina2,3, Douglas B. Sawyer4, Cristi L. Galindo2,3 1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2Department of Medicine, Vanderbilt University Medical Center, 3Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 4Cardiovascular Institute, Maine Medical Center Shown here is a method for visualizing extracellular matrix ultrastructure in decellularized cardiac tissues. Biology Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney Aaron C. Brown1, Ulrika Blank2, Derek C. Adams1, Michele J. Karolak1, Jennifer L. Fetting1, Beth L. Hill1, Leif Oxburgh1 1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.
