Queensborough Community College View Institution's Website 1 article published in JoVE Biology Measuring Fast Calcium Fluxes in Cardiomyocytes Urszula Golebiewska1, Suzanne Scarlata2 1Department of Biological Sciences and Geology, Queensborough Community College, 2Department of Physiology & Biophysics, Stony Brook University We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.