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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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University of Rochester

23 articles published in JoVE

 JoVE Neuroscience

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining


JoVE 50339 4/24/2013

,

Department of Biomolecular Genetics, University of Rochester Medical Center

Here we describe two assays that have been established to study age-dependent neurodegeneration of dopaminergic (DA) neurons in Drosophila: the climbing/startle-induced negative geotaxis assay which allows to study the functional effects of DA neurons degeneration and the tyrosine hydroxylase immunostaining which is used to identify and count DA neurons in whole brain mounts.

 JoVE Bioengineering

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy


JoVE 50163 2/21/2013

,

Department of Biomedical Engineering, University of Rochester

The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.

 JoVE Clinical and Translational Medicine

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis


JoVE 50124 12/28/2012

1, 2, 1, 3

1Orvis School of Nursing, University of Nevada, Reno, 2The State University of New York at Buffalo, St. Joseph's Medical Center, 3Strong Memorial Hospital, University of Rochester Medical Center

Continuous 12-lead electrocardiographic (ECG) monitoring can identify transient myocardial ischemia, even when asymptomatic, among patients with suspected acute coronary syndrome (ACS). In this article we describe our method for initiating patient monitoring using a Holter device, downloading the ECG data for off-line analysis, and how to utilize the ECG software to identify transient ischemia.

 JoVE Neuroscience

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence


JoVE 3466 2/21/2012

1, 2, 2, 1, 1, 3

1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Center for Neural Development and Disease, University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

 JoVE Bioengineering

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium


JoVE 3478 12/16/2011

1, 1, 2, 3

1Department of Chemistry, Duke University, 2Hajim School of Engineering and Applied Sciences, University of Rochester, 3Department of Chemical Engineering, University of Rochester

Here we describe a simple method for patterning oxide-free silicon and germanium with reactive organic monolayers and demonstrate functionalization of the patterned substrates with small molecules and proteins. The approach completely protects surfaces from chemical oxidation, provides precise control over feature morphology, and provides ready access to chemically discriminated patterns.

 JoVE Clinical and Translational Medicine

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse


JoVE 3039 7/25/2011

1, 2, 3

1Department of Pharmacology and Physiology, University of Rochester, 2Department of Neurology, University of Alabama at Birmingham, 3Departments of Anesthesiology, Pharmacology and Physiology, University of Rochester

Middle cerebral artery (MCA) ligation is a technique to study focal cerebral ischemia in animal models. In this method, the middle cerebral artery is exposed by craniotomy and ligated by cauterization. This method gives highly reproducible infarct volumes and increased post-operative survival rates compared to other methods available.

 JoVE Immunology and Infection

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci


JoVE 2512 1/25/2011

1, 1,2, 3, 1,4

1Center for Oral Biology, University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases, Sichuan University, 3Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology, University of Rochester Medical Center

Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.

 JoVE Neuroscience

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation


JoVE 2059 9/19/2010

*1, *2, 1, 2, 1

1Center for Neural Development and Disease, Department of Neurology, Child Neurology Division, University of Rochester, 2Department of Neurobiology and Anatomy, University of Rochester

We describe a method for repeatedly visualizing murine microglia and circulating monocytes in vivo over hours, days or weeks using transcranial two-photon microscopy. We demonstrate how to prepare a thinned-skull window that allows intermittent observation of quiescent microglia that can be activated by adjacent stereotactic injection of the HIV-1 regulatory protein Tat.

 JoVE Immunology and Infection

Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis


JoVE 2026 9/16/2010

, , ,

Department of Microbiology and Immunology, University of Rochester

The frog Xenopus laevis provides an attractive alternative non-mammalian model for exploring the ability of heat shock protein such as gp96 to promote antigen-specific CD8 T cell responses. We present methods to study in vivo facilitation of cross-presentation of skin and tumor antigens by gp96.

 JoVE Neuroscience

Double Fluorescence in situ Hybridization in Fresh Brain Sections


JoVE 2102 8/14/2010

1, 1, 1, 1,2

1Department of Brain and Cognitive Sciences, University of Rochester, 2Center for Visual Science, University of Rochester

This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

 JoVE Clinical and Translational Medicine

Murine Echocardiography and Ultrasound Imaging


JoVE 2100 8/08/2010

1, 2, 3, 2,4

1Department of Pharmacology and Physiology, University of Rochester, 2Aab Cardiovascular Research Institute, University of Rochester, 3Visualsonics, 4Department of Medicine, University of Rochester

This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.

 JoVE Immunology and Infection

Generation of Recombinant Influenza Virus from Plasmid DNA


JoVE 2057 8/03/2010

1, 2

1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine

Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.

 JoVE Neuroscience

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy


JoVE 2021 7/20/2010

1, 2, 1

1Department of Neurobiology and Anatomy, University of Rochester, 2Douglas Mental Health University Institute

We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

 JoVE General

In-vivo Centrifugation of Drosophila Embryos


JoVE 2005 6/23/2010

,

Department of Biology, University of Rochester

We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.

 JoVE General

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching


JoVE 1636 2/26/2010

1, 2

1Department of Physics and Astronomy, University of Rochester, 2Department of Biomedical Engineering, University of Rochester

In this article we will describe the procedure for measuring diffusion coefficients using multi-photon fluorescence recovery after photobleaching. We will begin by aligning the laser along the optical path to the sample and determining the proper experimental parameters, then continue generating and finally fitting fluorescence recovery curves.

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