University of Texas Medical Branch

3 articles published in JoVE

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

JoVE 50308 4/10/2013

Deborah R. Boone, Stacy L. Sell, Helen Lee Hellmich

Department of Anesthesiology, University of Texas Medical Branch

We describe how to use laser capture microdissection (LCM) to obtain enriched populations of hippocampal neurons or single neurons from frozen sections of the injured rat brain for subsequent gene expression analysis using quantitative real time PCR and/or whole-genome microarrays.

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

JoVE 4429 12/02/2012

Allison Poussard*, Michael Patterson*, Katherine Taylor, Alexey Seregin, Jeanon Smith, Jennifer Smith, Milagros Salazar, Slobodan Paessler

Experimental Pathology, University of Texas Medical Branch

Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study.

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

JoVE 4204 8/28/2012

Amina El Ayadi¹, Emily S. Stieren², José M. Barral², Andres F. Oberhauser², Darren Boehning²

¹Department of Surgery, University of Texas Medical Branch, ²Department of Neuroscience and Cell Biology, University of Texas Medical Branch

A method for large-scale purification of the APP intracellular domain (AICD) is described. We also describe methodology to induce in vitro AICD aggregation and visualization by atomic force microscopy. The methods described are useful for biochemical/structural characterization of the AICD and the effects of molecular chaperones on its aggregation.