Benaroya Research Institute View Institution's Website 2 articles published in JoVE Immunology and Infection Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs Maran L. Sprouse1, Gabriele Blahnik2, Thomas Lee1, Natalie Tully1, Pinaki Benarjee3, Eddie A. James2, Maria J. Redondo4, Matthew L. Bettini1, Maria Bettini1 1 The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression. Biology Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers Eddie A. James1, Rebecca LaFond2, Ivana Durinovic-Bello3, William Kwok2 1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.