JoVE   
You have subscription access to articles in this section through JoVE.

  JoVE Biology

  
You have subscription access to articles in this section through JoVE.

  JoVE Neuroscience

  
You have subscription access to articles in this section through JoVE.

  JoVE Immunology and Infection

  
You have subscription access to articles in this section through JoVE.

  JoVE Clinical and Translational Medicine

  
You have subscription access to articles in this section through JoVE.

  JoVE Bioengineering

  
You have subscription access to articles in this section through JoVE.

  JoVE Applied Physics

  
You have subscription access to articles in this section through JoVE.

  JoVE Chemistry

  
You have subscription access to articles in this section through JoVE.

  JoVE Behavior

  
You have subscription access to articles in this section through JoVE.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You have subscription access to videos in this collection through JoVE.

Basic Methods in Cellular and Molecular Biology

You have subscription access to videos in this collection through JoVE.

Model Organisms I

You have subscription access to videos in this collection through JoVE.

Model Organisms II

You have subscription access to videos in this collection through JoVE.

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by section
Biology
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
Behavior
Environment
 
 
 JoVE Biology

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

1Department of Biology, KU Leuven


JoVE 51516

The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).

 JoVE Neuroscience

Laser Nanosurgery of Cerebellar Axons In Vivo

1European Laboratory for Non-Linear Spectroscopy, University of Florence, 2National Institute of Optics, National Research Council, 3Department of Physics and Astronomy, University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)


JoVE 51371

Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.

 JoVE Neuroscience

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

1Veterans Administration Medical Center, San Diego, 2Department of Neurosciences, University of California, San Diego


JoVE 50641

Fibrin matrices containing growth factors were used to retain grafted neural stem cells into sites of complete spinal cord transection. Grafted cells completely filled the lesion cavity and differentiated into multiple neural cell types, including neurons that extended axons into host spinal cord over long distances.

 JoVE Biology

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam

1Department of Engineering Sciences, Uppsala University, 2Gatan Inc., 3Department of Microbiology, Swedish University of Agricultural Sciences, 4Physics Department, University of Oslo


JoVE 51463

Cryo Electron Microscopes, either Scanning (SEM) or Transmission (TEM), are widely used for characterization of biological samples or other materials with a high water content1. A SEM/Focused Ion Beam (FIB) is used to identify features of interest in samples and extract a thin, electron-transparent lamella for transfer to a cryo-TEM.

 JoVE Biology

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

1Department of Medical Biophysics, University of Toronto, 2Platform Biological Sciences, Sunnybrook Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Physical Sciences, Sunnybrook Research Institute, 5Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 6Manitoba Institute of Cell Biology, University of Manitoba


JoVE 51325

We present an image registration approach for 3-dimensional (3D) histology volume reconstruction, which facilitates the study of the changes of an organ at the level of macrostructures made up of cells . Using this approach, we studied the 3D changes between wild-type and Igfbp7-null mammary glands.

 JoVE Chemistry

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

1Chemical Sensing & Fuel Technology, Chemistry Division, U.S. Naval Research Laboratory, 2NOVA Research, Inc., 3Bio/Analytical Chemistry, Chemistry Division, U.S. Naval Research Laboratory, 4Navy Technology Center for Safety and Survivability, Chemistry Division, U.S. Naval Research Laboratory


JoVE 51938

Trace explosive vapors of TNT and RDX collected on sorbent-filled thermal desorption tubes were analyzed using a programmed temperature desorption system coupled to GC with an electron capture detector. The instrumental analysis is combined with direct liquid deposition method to reduce sample variability and account for instrumentation drift and losses.

 JoVE Bioengineering

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

1Institute of Pharmacology and Toxicology and Bio-Imaging Center/Rudolf Virchow Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Germany


JoVE 51784

This protocol describes how to use total internal reflection fluorescence microscopy to visualize and track single receptors on the surface of living cells and thereby analyze receptor lateral mobility, size of receptor complexes as well as to visualize transient receptor-receptor interactions. This protocol can be extended to other membrane proteins.

 JoVE Immunology and Infection

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

1Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel


JoVE 51724

Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.

 JoVE Neuroscience

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

1NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health


JoVE 51519

Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.

 JoVE Biology

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

1Laboratoire Matière et Systèmes Complexes, UFR de Physique, Université Paris Diderot, 2Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon


JoVE 51317

We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.

More Results...
Waiting
simple hit counter