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 JoVE Immunology and Infection

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey


JoVE 51806

DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.

 JoVE Immunology and Infection

Isolation of Murine Lymph Node Stromal Cells

1Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel


JoVE 51803

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

 JoVE Immunology and Infection

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

1Department of Genetics and Biochemistry, Clemson University Eukaryotic Pathogens Innovation Center


JoVE 51647

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

 JoVE Neuroscience

A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation

1Division of Biomedical Sciences, Faculty of Medicine, Memorial University, 2Division of Medical Sciences, University of Victoria


JoVE 51808

This protocol introduces lateralized early odor preference learning in rats using acute single naris occlusion. Lateralized learning permits the examination of behavioral outcomes and underpinning biological mechanisms within the same animals, reducing variance induced by between-animal designs. This protocol can be used to investigate molecular mechanisms underpinning early odor learning.

 JoVE Clinical and Translational Medicine

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

1Department of Medicine, Renal division, University of Pittsburgh School of Medicine, 2Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine


JoVE 51807

This manuscript presents a simple, yet powerful, in vitro method for evaluating smooth muscle contractility in response to pharmacological agents or nerve stimulation. Main applications are drug screening and understanding tissue physiology, pharmacology, and pathology.

 JoVE Neuroscience

Subtype-selective Electroporation of Cortical Interneurons

1NYU Neuroscience Institute, New York University School of Medicine, 2Brain and Mind Research Institute, Weill Cornell Medical College


JoVE 51518

This procedure shows how to target interneurons in the developing mouse forebrain by means of in utero electroporation. This technique was particularly efficient to achieve selective gene expression in interneuron subtypes destined to the superficial layers of the cortex.

 JoVE Biology

Massively Parallel Reporter Assays in Cultured Mammalian Cells

1Broad Institute


JoVE 51719

The genetic reporter assay is a well-established and powerful tool for dissecting the relationship between DNA sequences and their gene regulatory activities. Coupling candidate regulatory elements to reporter genes that carry identifying sequence tags enables massive parallelization of these assays.

 JoVE Neuroscience

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson's Disease Model of Drosophila

1Department of Biology, Colby College


JoVE 51625

Parkinson’s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, causing locomotion defects. Rotenone models Parkinson’s disease in Drosophila. This paper outlines two assays that characterize both spontaneous and startle-induced locomotion deficiencies caused by rotenone.

 JoVE Bioengineering

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

1Biomedical Engineering, Wayne State University


JoVE 51517

This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.

 JoVE Neuroscience

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

1Vascular Biology Program, Boston Children's Hospital, 2Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, 3Department of Ophthalmology, Harvard Medical School


JoVE 51375

The protocol describes the corneal micropocket assay as developed in mice.

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