JoVE Clinical and Translational Medicine
1Department of Biological Sciences, Purdue University, 2Department of Oncology, University of Alberta, 3Cross Cancer Institute
In standard culture methods cells are taken out of their physiological environment and grown on the plastic surface of a dish. To study the behavior of primary human bone marrow cells we created a 3-D culture system where cells are grown under conditions recapitulating the native microenvironment of the tissue.
Published March 8, 2014. Keywords: Medicine, extracellular matrix, 3D culture, bone marrow, hematological malignancies, primary cell culture, tumor microenvironment
1Department of Otolaryngology, Morsani College of Medicine, University of South Florida
Proteome analysis of the cochlear sensory epithelium can be challenging due to its small size and because membrane proteins are difficult to isolate and identify. Both membrane and soluble proteins can be identified by combining multiple preparative methods and separation techniques along with high-resolution mass spectrometry.
Published March 7, 2014. Keywords: Biochemistry, Cochlear, chromatography, LC-MS/MS, mass spectrometry, Proteomics, sensory epithelium
1Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, 2Department of Radiology and Biomedical Imaging, University of California San Francisco, 3Xradia Inc.
In this study, the use of an in situ loading device coupled with micro-X-ray computed tomography for fibrous joint biomechanics will be discussed. Experimental readouts identifiable with an overall change in joint biomechanics will include: 1) reactionary force vs. displacement, i.e. tooth displacement within the alveolar socket and its reactionary response to loading, 2) three-dimensional (3D) spatial configuration and morphometrics, i.e. geometric relationship of the tooth with the alveolar socket, and 3) changes in readouts 1 and 2 due to a change in loading axis, i.e. concentric or eccentric loads.
Published March 7, 2014. Keywords: Bioengineering, biomechanics, bone-periodontal ligament-tooth complex, concentric loads, eccentric loads, contrast agent
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh
This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.
Published March 7, 2014. Keywords: Bioengineering, Receptors, Cell Surface, Polymers, Cell Adhesion, Biomedical and Dental Materials, parylene-C, silicon dioxide, photolithography, cell adhesion, Cell Patterning
1Annis Water Resources Institute, Grand Valley State University
Lake eutrophication is a water quality issue worldwide, making the need to identify and control nutrient sources critical. Laboratory determination of phosphorus release rates from sediment cores is a valuable approach for determining the role of internal phosphorus loading and guiding management decisions.
Published March 6, 2014. Keywords: Environmental Sciences, Limnology, internal loading, eutrophication, nutrient flux, sediment coring, phosphorus, lakes
JoVE Clinical and Translational Medicine
1Radiology, University of Texas Southwestern Medical Center
A breast cancer brain metastasis mouse model is established with ultrasound imaging-guided intracardiac injection of MDA-MB231/Br-GFP cells. Development of multifocal intracranial metastases has been monitored longitudinally using high-resolution 9.4 T MRI.
Published March 6, 2014. Keywords: Medicine, breast cancer brain metastasis, intracardiac injection, ultrasound imaging, MRI, MDA-MB231/Br-GFP cells
1Department of Physics, University of Ottawa, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3Centre for Neural Dynamics, University of Ottawa
We describe a set of techniques for studying spontaneous behavior of freely swimming weakly electric fish over an extended period of time, by synchronously measuring the animal's electric organ discharge timing, body position and posture both accurately and reliably in a specially designed aquarium tank inside a sensory isolation chamber.
Published March 6, 2014. Keywords: Neuroscience, animal tracking, weakly electric fish, electric organ discharge, underwater infrared imaging, automated image tracking, sensory isolation chamber, exploratory behavior
1Department of Chemistry, University of Wisconsin- Madison, 2School of Pharmacy, University of Wisconsin- Madison
Mass spectrometric imaging (MSI) is a powerful tool that can be used to discover and identify various chemical species in intact tissues, preserving the compounds in their native environments, which can provide new insights into biological processes. Herein a MSI method developed for the analysis of small molecules is described.
Published March 5, 2014. Keywords: Basic Protocol, Mass Spectrometric Imaging, Imaging Mass Spectrometry, MALDI, TOF/TOF, Medicago truncatula, Metabolite, Small Molecule, Sublimation, Automatic Sprayer
1Conrad N. Hilton College of Hotel and Restaurant Management, University of Houston, 2Center for Food Safety, Department of Food Science, University of Arkansas
The overall goal of this study was to demonstrate the potential cross contamination mechanism of foodborne pathogen Listeria monocytogenes in a retail deli setting. This methodology may be applied to a variety of different environments to track pathogen contamination.
Published March 5, 2014. Keywords: Environmental Sciences, cross contamination, retail deli, fluorescent powder, Listeria monocytogenes, foodborne pathogens
JoVE Applied Physics
1Department of Physics, The University of Chicago, 2James Franck Institute, The University of Chicago, 3Department of Mechanical Engineering and Materials Science, Yale University
Drop impact of non-Newtonian fluids is a complex process since different physical parameters influence the dynamics over a very short time (less than one tenth of a millisecond). A fast imaging technique is introduced in order to characterize the impact behaviors of different non-Newtonian fluids.
Published March 5, 2014. Keywords: Physics, fluid mechanics, fast camera, dense suspension, liquid metal, drop impact, splashing