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 JoVE Biology

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

1Department of Oncological Sciences, University of Utah


JoVE 51060

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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 JoVE Bioengineering

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

1Albrecht-Kossel-Institute for Neuroregeneration, University of Rostock


JoVE 3830

Here we describe the use of a self-assembling 3-dimensional scaffold to culture human neural progenitor cells. We present a protocol to release the cells from the scaffolds to be analysed subsequently e.g. by flow cytometry. This protocol might be adapted to other cell types to perform detailed mechanistically studies.

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 JoVE Biology

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

1Medical Biochemistry and Molecular Biology, Saarland University, 2Experimental and Clinical Pharmacology and Toxicology, Saarland University


JoVE 2730

The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.

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 JoVE Biology

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

1Department of Neurology, University of California, Los Angeles


JoVE 678

Cortical blood flow dynamics can be studied in vivo by imaging fluorescent dextran dyes injected into the tail vein of rodents with 2-photon microscopy. This video shows how to image blood flow dynamics in neocortex of mice through a glass-covered cranial window preparation.

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 JoVE Biology

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology


JoVE 734

This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

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 JoVE Biology

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

1David Geffen School of Medicine, University of California, Los Angeles


JoVE 831

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.

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 JoVE Biology

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

1David Geffen School of Medicine, University of California, Los Angeles


JoVE 832

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 3 of 3.

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 JoVE Biology

Calcium Imaging of Cortical Neurons using Fura-2 AM

1Department of Neurobiology, Stanford University, 2Department of Neurobiology, Stanford University School of Medicine


JoVE 1067

Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

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 JoVE Biology

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

1Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology


JoVE 1169

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 2 of 3.

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 JoVE Biology

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

1Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology


JoVE 1170

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.

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