JoVE Clinical and Translational Medicine
1Boston Scientific , Neuromodulation
Using a mathematical model of spinal cord stimulation, we found that a multi-source system with independent power sources for each contact can target more central points of stimulation on the dorsal column (100 vs 3) and has 50-fold more field steering resolution (0.02mm vs 1mm) than a single-source system.
Published February 10, 2011. Keywords: Medicine, spinal cord stimulation, dorsal columns, current steering, field steering
1CGphiMC, UMR CNRS 5534, University of Lyon 1
The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.
Published February 11, 2014. Keywords: Neuroscience, Neurons, Growth Cones, Axons, Embryonic Development, Floor plate, conditioned medium, axon guidance, commissural tissue, biochemistry, receptor levels
JoVE Immunology and Infection
1Department of Laboratory Medicine and Pathology, Mayo Clinic
Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.
Published June 24, 2011. Keywords: Immunology, Mycobacterium tuberculosis, MIC, antimicrobial susceptibility testing, first and second line drugs, microtiter plate, broth dilution
1Department of Biomedical Engineering, McGill University, Montreal, QC Canada
Nanoparticles such as semiconductor quantum dots (QDs) can be used to create photoactivatable agents for anti-microbial or anti-cancer applications. This technique shows how to water-solubilize cadmium telluride (CdTe) QDs, conjugate them to an antibiotic, and perform a bacterial inhibition assay based upon growth curves and plate count.
Published July 11, 2012. Keywords: Biomedical Engineering, Bioengineering, Molecular Biology, Quantum dots, solubilization, conjugation, cytotoxicity, phototoxicity, growth curve, plate count
1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster
Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.
Published March 5, 2013. Keywords: Stem Cell Biology, Neuroscience, Biomedical Engineering, Medicine, Bioengineering, Physiology, Genetics, Molecular Biomedicine, human pluripotent stem cells, hPSC, neuronal differentiation, neuroectoderm, embryoid bodies, chemically defined conditions, stem cells, neurons, signalling pathways, mid-throughput, PCR, multititre, cell culture
1Department of Internal Medicine, Division of Geriatric Medicine, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan
Here we present a protocol for performing solid plate-based dietary restriction in C. elegans with killed bacteria.
Published May 28, 2011. Keywords: Developmental Biology, Dietary restriction, caloric restriction, C. elegans, longevity
1Department of Chemistry, Drexel University, 2Mettler-Toledo, 3Chemspider
The Ugi reaction has proved to be a convenient way to quickly create diverse libraries of compounds. It involves the reaction of an amine, an aldehyde, a carboxylic acid and an isonitrile typically in methanol at room temperature. In this video, we utilize a 48-slot Mettler-Toledo MiniBlock equipped with filtration tubes and a Mettler-Toledo MiniMapper automated liquid handler was used to deliver the reagents and solvent. The parameters of interest were the concentration, the solvent composition and the excess of some of the reagents.
Published November 11, 2008. Keywords: Chemistry, Ugi Reaction, Automated Liquid Handling, Combinatorial Chemistry, organic chemistry, Mini-block, Open Notebook Science, reaction optimization, UsefulChem, MiniBlock, precipitate
JoVE Immunology and Infection
1Department of Biology, University of Texas San Antonio - UTSA, 2South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio - UTSA
We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.
Published October 21, 2010. Keywords: Immunology, Microbiology, Medical Mycology, Candida, candidiasis, biofilms, antifungals
JoVE Application Notes
The IonFlux Automated Patch Clamp System provides high throughput, cost-effective ion channel screening for a wide range of electrophysiology applications. Fast compound exchange, low cost per data point, and convenient well plate formats make the system ideal for both ligand- and voltage-gated ion channel targets. The IonFlux HT provides an industry-leading 10,000 data points per day, while the IonFlux 16 provides true automated patch clamp performance for about the cost of a manual patch clamp rig.
Published September 15, 2010. Keywords: Ion channel, patch clamp, electrophysiology, Fluxion Biosciences, IonFlux, high throughput screening, cell based assay
JoVE Immunology and Infection
1Protein Crystallization Lab, Emerald Bio, 2Molecular Biology Lab, Emerald Bio, 3Scientific Sales Representative, Emerald Bio, 4Group Leader II, Emerald Bio, 5Group Leader I, Emerald Bio, 6Chair of Advisory Board, Emerald Bio, 7Director of Multi-Target Services, Emerald Bio, 8Senior Project Leader, Emerald Bio, 9Project Leader II & SSGCID Site Manager, Emerald Bio
Structure-based drug design plays an important role in drug development. Pursuing multiple targets in parallel greatly increases the chance of success for lead discovery. The following article highlights how the Seattle Structural Genomics Center for Infectious Disease utilizes a multi-target approach for gene-to-structure determination of the PB2 influenza A subunit.
Published June 28, 2013. Keywords: Infection, Structural Biology, Virology, Genetics, Medicine, Biomedical Engineering, Molecular Biology, Infectious Diseases, Microbiology, Genomics, high throughput, multi-targeting, structural genomics, protein crystallization, purification, protein production, X-ray crystallography, Gene Composer, Protein Maker, expression, E. coli, fermentation, influenza, virus, vector, plasmid, cell, cell culture, PCR, sequencing