Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

JoVE 3830 1/11/2012

Andrea Liedmann, Arndt Rolfs, Moritz J. Frech

Albrecht-Kossel-Institute for Neuroregeneration, University of Rostock

Here we describe the use of a self-assembling 3-dimensional scaffold to culture human neural progenitor cells. We present a protocol to release the cells from the scaffolds to be analysed subsequently e.g. by flow cytometry. This protocol might be adapted to other cell types to perform detailed mechanistically studies.