Isolation of Immune Cells from Primary Tumors

JoVE 3952 6/16/2012

Stephanie K. Watkins¹, Ziqiang Zhu¹, Keith E. Watkins², Arthur A. Hurwitz¹

¹Tumor Immunity and Tolerance Section, Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, National Cancer Institute - Frederick, ²KEWB Productions

In this report, we describe a protocol for isolating highly purified populations of leukocytes that infiltrate tumors. This protocol is adapted from the Miltenyi Biotech protocol to enhance yield and purity for isolating cells from complex tumor tissue.