bioengineering in JoVE

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

JoVE 1650 12/14/2009

Onur Mudanyali¹, Anthony Erlinger¹, Sungkyu Seo¹, Ting-Wei Su¹, Derek Tseng¹, Aydogan Ozcan^1,2

¹Electrical Engineering Department, University of California, Los Angeles, ²California NanoSystems Institute, University of California, Los Angeles

Lensfree on-chip imaging and characterization of cells is illustrated. This on-chip cell imaging approach provides a compact and cost-effective tool for medical diagnostics and high-throughput cell biology applications, making it especially suitable for resource poor settings.

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

JoVE 3288 2/07/2012

Christophe Py¹, Marzia Martina², Robert Monette², Tanya Comas², Mike W. Denhoff¹, Collin Luk³, Naweed I. Syed³, Geoff Mealing²

¹Institute for Microstructural Sciences, National Research Council of Canada, ²Institute for Biological Sciences, National Research Council of Canada, ³Hotchkiss Brain Institute, University of Calgary

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

JoVE 4023 3/15/2012

Thomas Wucherpfennig, Antonia Lakowitz, Habib Driouch, Rainer Krull, Christoph Wittmann

Institute of Biochemical Engineering, Technische Universität Braunschweig

A method to precisely generate and to comprehensively characterize morphology of filamentous fungus Aspergillus niger is described, which allows the mathematical correlation of morphological appearance and productivity.

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

JoVE 1590 10/26/2009

Sudhir Khetan¹, Jason Burdick²

¹Department of Bioengineering, University of Pennsylvania , ²Department of Bioengineering, University of Pennsylvania-School of Medicine

We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

JoVE 2184 11/18/2010

Pedro Cabrales¹, Leonardo J. M. Carvalho²

¹Bioengineering, University of California, San Diego, ²La Jolla Bioengineering Institute

Intravital microscopy to follow temporal and spatial hemodynamic and inflammatory events in the pial microcirculation.

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

JoVE 296 10/01/2007

Nianzhen Li, Chris Sip, Albert Folch

Dept. of Bioengineering, University of Washington

We demonstrate protocols for manufacturing and automating elastomeric polydimethylsiloxane (PDMS)-based microvalve arrays that need no extra energy to close and feature photolithographically defined precise volumes. A parallel subnanoliter-volume mixer and an integrated microfluidic perfusion system are presented.