JoVE   
You have trial access to articles in this section through JoVE.

  JoVE Biology

  
You have trial access to articles in this section through JoVE.

  JoVE Neuroscience

  
You have trial access to articles in this section through JoVE.

  JoVE Immunology and Infection

  
You have subscription access to articles in this section through JoVE.

  JoVE Clinical and Translational Medicine

  
You have subscription access to articles in this section through JoVE.

  JoVE Bioengineering

  
You have subscription access to articles in this section through JoVE.

  JoVE Applied Physics

  
You have subscription access to articles in this section through JoVE.

  JoVE Chemistry

  
You have subscription access to articles in this section through JoVE.

  JoVE Behavior

  
You have subscription access to articles in this section through JoVE.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You have subscription access to videos in this collection through JoVE.

Basic Methods in Cellular and Molecular Biology

You have subscription access to videos in this collection through JoVE.

Model Organisms I

You have subscription access to videos in this collection through JoVE.

Model Organisms II

You have subscription access to videos in this collection through JoVE.

Essentials of
Neuroscience

You have subscription access to videos in this collection through JoVE.

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by section
Biology
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
Behavior
Environment
 
 
 JoVE Immunology and Infection

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

1Department of Anesthesia and Perioperative Care, University of California, San Francisco, 2Graduate Program in Biomedical Sciences, University of California, San Francisco


JoVE 50677

Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.

 JoVE Biology

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH


JoVE 51312

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

 JoVE Immunology and Infection

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

1Division of Pediatrics, MD Anderson Cancer Center - University of Texas


JoVE 2540

Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.

 JoVE Clinical and Translational Medicine

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

1Institute of Human Physiology and Clinical Experimental Research, Semmelweis University


JoVE 3575

We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.

 JoVE Bioengineering

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

1School of Engineering, University of California, Merced


JoVE 51044

For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite. This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.

 JoVE Clinical and Translational Medicine

Modeling and Imaging 3-Dimensional Collective Cell Invasion

1Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, 2The Beatson Institute for Cancer Research


JoVE 3525

Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.

 JoVE Immunology and Infection

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

1Department of Immunology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine


JoVE 4227

Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.

 JoVE Bioengineering

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

1School of Biological and Health Systems Engineering, Arizona State University


JoVE 4415

The article details the protocol for site-specific transfection of scrambled sequence of siRNA in an adherent mammalian cell culture using a microelectrode array (MEA).

 JoVE Chemistry

Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

1Department of Biomedical Engineering, University of Rochester, 2Department of Chemical Engineering, University of Rochester, 3Center for Musculoskeletal Research, University of Rochester Medical Center


JoVE 50890

This video will illustrate a rapid, efficient method to methacrylate poly(ethylene glycol), enabling chain polymerizations and hydrogel synthesis. It will demonstrate how to similarly introduce methacrylamide functionalities into peptides, detail common analytical methods to assess functionalization efficiency, provide suggestions for troubleshooting and advanced modifications, and demonstrate typical hydrogel characterization techniques.

More Results...
Waiting
simple hit counter