JoVE   
You have subscription access to articles in this section through JoVE.

  JoVE Biology

  
You have subscription access to articles in this section through JoVE.

  JoVE Neuroscience

  
You have subscription access to articles in this section through JoVE.

  JoVE Immunology and Infection

  
You have subscription access to articles in this section through JoVE.

  JoVE Clinical and Translational Medicine

  
You have subscription access to articles in this section through JoVE.

  JoVE Bioengineering

  
You have subscription access to articles in this section through JoVE.

  JoVE Applied Physics

  
You have subscription access to articles in this section through JoVE.

  JoVE Chemistry

  
You have subscription access to articles in this section through JoVE.

  JoVE Behavior

  
You have subscription access to articles in this section through JoVE.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You have subscription access to videos in this collection through JoVE.

Basic Methods in Cellular and Molecular Biology

You have subscription access to videos in this collection through JoVE.

Model Organisms I

You have subscription access to videos in this collection through JoVE.

Model Organisms II

You have subscription access to videos in this collection through JoVE.

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by section
Biology
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
Behavior
Environment
 
 
Caspase 3: A short pro-domain caspase that plays an effector role in Apoptosis. It is activated by Initiator caspases such as Caspase 9. Isoforms of this protein exist due to multiple alternative splicing of its Messenger rna.
 JoVE Biology

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

1Department of Oncological Sciences, University of Utah


JoVE 51060

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

 JoVE Neuroscience

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota


JoVE 51305

Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.

 JoVE Neuroscience

Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization

1Department of Molecular and Cellular Biology, University of Guelph


JoVE 3422

Live-cell imaging of caspase-3 mediated apoptosis in immortalized N19-oligodendrocyte cell cultures using the NucView 488 caspase-3 substrate. This technique is applicable for programmed cell death assays in real-time in a variety of cell types and tissues.

 JoVE Neuroscience

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

1Neurodevelopment Group, School of Biosciences, University of Birmingham


JoVE 50306

An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

 JoVE Bioengineering

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

1Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University


JoVE 3612

Type B gelatin-based engineered nanovectors system (GENS) was developed for systemic gene delivery and transfection in the treatment of pancreatic cancer. By modification with epidermal growth factor receptor (EGFR) specific peptide on the surface of nanparticles, they could target on EGFR receptor and release plasmid under reducing environment, such as high intracellular glutathione concentrations.

 JoVE Bioengineering

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

1Pediatric Airway Research Center, Department of Pediatrics, University of Colorado, 2Department of Chemical and Biological Engineering, Colorado School of Mines


JoVE 51539

This protocol is designed to demonstrate exposure method of cell cultures to inhaled toxic chemicals. Exposure of differentiated air-liquid interface (ALI) cultures of airway epithelial cells provides a unique model of airway exposure to toxic gases such as chlorine. In this manuscript we describe effect of chlorine exposure on air-liquid interface cultures of epithelial cells and submerged culture of cardiomyocytes. In vitro exposure systems allow important mechanistic studies to evaluate pathways that could then be utilized to develop novel therapeutic agents.

 JoVE Clinical and Translational Medicine

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

1St. Erik's Eye Hospital, Karolinska Institutet, 2Gullstrand lab, Section for Ophthalmology, Department of Neuroscience, Uppsala University


JoVE 4016

Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.

 JoVE Immunology and Infection

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center


JoVE 51284

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

 JoVE Biology

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

1Department of Chemistry, Tunghai University, 2Department of Biochemistry, University of California, Riverside


JoVE 3602

MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

 JoVE Biology

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California


JoVE 4254

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

More Results...
Waiting
simple hit counter