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Opening soon: July 2012

JoVE is expanding its scope to include applied physics. We are now accepting technique based manuscripts for our inaugural issues. Open a window into your lab with a visual publication of your protocols.

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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells


JoVE 2645 5/31/2011

Rita Nokes Cook, Su Fen Ang*, Richard Seung-on Kang*, Heike Fölsch

Department of Cell and Molecular Biology, Northwestern University

This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.

 

October 2011: This Month in JoVE


JoVE 3957 10/01/2011

Aaron Kolski-Andreaco

 

July 2011: This Month in JoVE


JoVE 3688 7/01/2011

Aaron Kolski-Andreaco

 

September 2011: This Month in JoVE


JoVE 3877 9/01/2011

Aaron Kolski-Andreaco

 

December 2011: This Month in JoVE


JoVE 4114 12/01/2011

Aaron Kolski-Andreaco

 

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips


JoVE 3288 2/07/2012

Christophe Py1, Marzia Martina2, Robert Monette2, Tanya Comas2, Mike W. Denhoff1, Collin Luk3, Naweed I. Syed3, Geoff Mealing2

1Institute for Microstructural Sciences, National Research Council of Canada, 2Institute for Biological Sciences, National Research Council of Canada, 3Hotchkiss Brain Institute, University of Calgary

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

 

February 2012: This Month in JoVE


JoVE 4258 2/01/2012

Aaron Kolski-Andreaco

 

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells


JoVE 2383 1/15/2011

Martin Weiss Nielsen1, Claus Sternberg1, Søren Molin1, Birgitte Regenberg2

1Department of Systems Biology, Danish Technical University, 2Department of Biology, University of Copenhagen

Protocol describing the application of a flow cell system for growing and analyzing microbial biofilms for Confocal Laser Scanning Microscopy (CLSM).

 

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study


JoVE 2723 10/12/2011

Matthew Nienaber*, Jeong Soon Lee*, Ruqiang Feng, Jung Yul Lim

Department of Engineering Mechanics, University of Nebraska-Lincoln

A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate the molecular/cellular mechanisms of blast-induced traumatic brain injury.

 

Single-cell Profiling of Developing and Mature Retinal Neurons


JoVE 3824 4/19/2012

Jillian J. Goetz, Jeffrey M. Trimarchi

Department of Genetics, Development and Cell Biology, Neuroscience Program, Iowa State University

A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.

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