The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets


JoVE 3650 5/04/2012

Department of Molecular Microbiology, University of Texas Southwestern Medical Center

The CLEM technique has been adapted to analyze ultrastructural morphology of membranes, organelles, and subcellular structures affected by microinjected molecules. This method combines the powerful techniques of micromanipulation/microinjection, confocal fluorescent microscopy, and electron microscopy to allow millimeter to multi-nanometer resolution. This technique is amenable to a wide variety of applications.

 

Shape Memory Polymers for Active Cell Culture


JoVE 2903 7/04/2011

Department of Biomedical and Chemical Engineering, Syracuse Biomaterials Institute

A method for developing cell culture substrates with the ability to change topography during culture is described. The method makes use of smart materials known as shape memory polymers that have the ability to memorize a permanent shape. This concept is adaptable to a wide range of materials and applications.

 

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy


JoVE 2909 6/05/2011

Department of Molecular and Cellular Biology, University of California Davis

We demonstrate the fabrication of a low-cost cryogenic stage designed to fit most reflected light microscopes. This lab-built cryogenic stage enables efficient and reliable correlative imaging between cryo-light and cryo-electron microscopy.

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