Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

JoVE 2557 3/24/2011

Diane Hu, Ralph S. Marcucio

Department of Orthopaedic Surgery, University of California San Francisco

This article describes a tissue transplantation technique that was designed to test the signaling and patterning properties of surface cephalic ectoderm during craniofacial development.

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

JoVE 2159 10/27/2010

Gretchen Mahler, Russell Gould, Johnathan Butcher

Department of Biomedical Engineering, Cornell University

This article will provide a method for isolating and culturing quail or chicken HH14^- valve endocardial cells and HH25 valve cushion mesenchymal cells.

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

JoVE 2010 8/21/2010

Enrique Amaya¹, Kristen Kroll²

¹The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, ²Department of Developmental Biology, Washington University School of Medicine

This video protocol demonstrates a method for generating transgenic Xenopus laevis by introduction of transgenes into sperm nuclei followed by nuclear transplantation into unfertilized eggs.

Issue 9: Transplantation, Electroporation, and more...

JoVE 412 11/01/2007

Jove Team

JoVE

In situ Protocol for Butterfly Pupal Wings Using Riboprobes

JoVE 208 5/28/2007

Diane Ramos¹, Antonia Monteiro²

¹Department of Biological Sciences, SUNY-University at Buffalo, ²Dept. Ecology and Evolutionary Biology, Yale University

In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.

Isolation and Derivation of Mouse Embryonic Germinal Cells

JoVE 1635 10/22/2009

Harold Moreno-Ortiz, Clara Esteban-Perez, Wael Badran, Marijo Kent-First

Reproductive Genetics and Stem Cell Laboratory, Department of Biological Sciences, Mississippi State University

The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.

March 2012: This Month in JoVE

JoVE 4336 3/01/2012

Aaron Kolski-Andreaco

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

JoVE 2150 12/11/2010

Tadeusz J. Kaczynski, Shermali Gunawardena

Department of Biological Sciences, SUNY-University at Buffalo

We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis.

Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

JoVE 1940 7/03/2010

Maximiano P. Ribeiro, Ricardo Relvas, Samuel Chiquita, Ilídio J. Correia

Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior

The umbilical cords are used to isolate smooth muscle cells by different ways. In this work we used the enzymatic treatment to isolated smooth muscle cells.

Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

JoVE 1519 9/22/2009

Peggy S. Zelenka, Chun Y. Gao, Senthil S. Saravanamuthu

Laboratory of Molecular and Developmental Biology, National Eye Institute (NEI), National Institutes of Health (NIH)

Explants of the central region of rat lens epithelia differentiate synchronously when cultured in the presence of FGF-2. Immunofluorescence microscopy of such cultures can provides novel information about gene expression and signaling events associated with terminal differentiation.