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Estrous Cycle: The period of cyclic physiological and behavior changes in non-primate female mammals that exhibit Estrus. The estrous cycle generally consists of 4 or 5 distinct periods corresponding to the endocrine status (Proestrus; Estrus; Metestrus; Diestrus; and Anestrus).
 JoVE Biology

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

1Department of Biochemistry, Microbiology and Immunology, Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3CIHR Program in Neurodegenerative Lipidomics, University of Ottawa, 4Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism


JoVE 4389

 Science Education: Essentials of Lab Animal Research

Fundamentals of Breeding and Weaning

JoVE Science Education

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Millions of mice and rats are bred for use in biomedical research each year. Worldwide, there are several large commercial breeding facilities that supply mice to research laboratories, but many facilities choose to also breed mice and rats in-house to reduce costs and increase research options. When breeding in the animal facility, researchers are able to manipulate the genetics of the animals, time the pregnancies to meet the needs of the research, and work with embryos and neonates as required. Mice and rats can be bred in a variety of schemes and methods. Technical procedures, such as the use of vaginal cytology, visualization of the vaginal area, and observation of copulatory plugs, have been developed to assist with the synchronization of breeding to correspond to research requirements. This manuscript is an overview of the basic fundamentals of mouse and rat breeding and technical procedures used. More detailed descriptions of the complex breeding schemes, and the full description of the methods for vaginal cytology, are available in the list of references.

 JoVE Behavior

Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

1Department of Psychology, Behavioral Neuroscience, West Virginia University, 2Department of Physiology and Pharmacology, West Virginia University, 3Department of Neurology, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 4Department of Neuroscience, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 5GRECC, VA Medical Center, 6Center for Neuroscience, Center for Basic and Translational Stroke Research, West Virginia University


JoVE 52706

 JoVE Neuroscience

Non-restraining EEG Radiotelemetry: Epidural and Deep Intracerebral Stereotaxic EEG Electrode Placement

1Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices (Bundesinstitut für Arzneimittel und Medizinprodukte, BfArM), 2Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases (Deutsches Zentrum für Neurodegenerative Erkrankungen, DZNE)


JoVE 54216

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 JoVE Chemistry

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

1Institute for Critical Technology and Applied Science, Virginia Tech, 2Macromolecules and Interfaces Institute, Virginia Tech, 3Institute for Food Safety and Health, Illinois Institute of Technology- Moffett Campus, 4Wood, Cellulose, and Paper Research Department, University of Guadalajara, 5Department of Sustainable Biomaterials, Virginia Tech, 6Sustainable Nanotechnology Interdisciplinary Graduate Education Program, Virginia Tech


JoVE 51257

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 JoVE Neuroscience

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

1CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, 2INSERM, UMR 967, 3Université Paris Diderot, Sorbonne Paris Cité, UMR 967, 4Université Paris Sud, UMR 967, 5CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department


JoVE 53247

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