JoVE General
Large Insert Environmental Genomic Library Production
Department of Microbiology and Immunology, University of British Columbia - UBC
Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.
JoVE General
Large Volume (20L+) Filtration of Coastal Seawater Samples
Department of Microbiology and Immunology, University of British Columbia - UBC
This video documents large volume (≥20 L) filtration of microbial biomass, ranging between 0.22μm and 2.7μm in diameter, from the water column.
JoVE General
Seawater Sampling and Collection
Department of Microbiology and Immunology, University of British Columbia - UBC
This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.
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