Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

JoVE 3288 2/07/2012

Christophe Py¹, Marzia Martina², Robert Monette², Tanya Comas², Mike W. Denhoff¹, Collin Luk³, Naweed I. Syed³, Geoff Mealing²

¹Institute for Microstructural Sciences, National Research Council of Canada, ²Institute for Biological Sciences, National Research Council of Canada, ³Hotchkiss Brain Institute, University of Calgary

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging

JoVE 3236 9/11/2011

Steve Burion, Tobias Funk

Triple Ring Technologies

We are developing a dynamic adaptive exposure technique using our scanning beam digital X-ray system. Rather than exposing an object uniformly, the exposure is adapted depending on the opacity of the object. Here we show an experiment on an anthropomorphic phantom that resulted in a dose saving of 30%.

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

JoVE 2097 9/03/2010

Peter Nemes, Akos Vertes

Department of Chemistry, George Washington University

Laser ablation electrospray ionization (LAESI) is an atmospheric-pressure ion source for mass spectrometry. In the imaging mode, a mid-infrared laser probes the distributions of molecules across a tissue section or a biofilm. This technique presents a new approach for diverse bioanalytical studies carried out under native experimental conditions.

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

JoVE 2688 6/21/2011

Amnon Sharir¹, Gregory Ramniceanu², Vlad Brumfeld³

¹Department of Molecular Genetics, Weizmann Institute of Science, ²Department of Biological Regulation, Weizmann Institute of Science, ³Department of Chemical Infrastructure, Weizmann Institute of Science

Non-destructive volume visualization can be achieved only by tomographic techniques, of which the most efficient is the x-ray micro computerized tomography ( CT).

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

JoVE 2144 9/04/2010

Bindesh Shrestha, Akos Vertes

Department of Chemistry, George Washington University

Single cell analysis is performed by mass spectrometry on plant and animal cells at atmospheric pressure by using a sharpened optical fiber to sample the cells for laser ablation electrospray ionization (LAESI) mass spectrometry.

Assessment of Motor Balance and Coordination in Mice using the Balance Beam

JoVE 2376 3/10/2011

Tinh N. Luong*, Holly J. Carlisle*, Amber Southwell, Paul H. Patterson

Department of Biology, California Institute of Technology

Deficits in fine motor coordination can be assessed with the balance beam test. Performance on the beam is quantified by the speed at which the beam is traversed and the number of times the mouse slips on the beam.

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JoVE 2268 6/15/2010

Robert S. Plumb

Pharmaceutical Business Operations, Waters Corporation

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

JoVE 1985 7/31/2010

Izhak Michaelevski, Noam Kirshenbaum, Michal Sharon

Department of Biological Chemistry, Weizmann Institute of Science

Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.