A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke

JoVE 3406 1/02/2012

Long-Xi Yu¹, Boris G. Dzikovski², Jack H. Freed²

¹CDCF-AOX Lab, ²National Biomedical Center for Advanced ESR Technology (ACERT), Department of Chemistry and Chemical Biology, Cornell University

Spin-trapping ESR spectroscopy was used to study the effect of plant antioxidants lycopene, pycnogenol and grape seed extract on scavenging gas-phase free radicals in cigarette smoke.

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

JoVE 1590 10/26/2009

Sudhir Khetan¹, Jason Burdick²

¹Department of Bioengineering, University of Pennsylvania , ²Department of Bioengineering, University of Pennsylvania-School of Medicine

We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.

January 2012: This Month in JoVE

JoVE 4194 1/02/2012

Aaron Kolski-Andreaco

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

JoVE 3357 11/21/2011

Danli Wu, Patricia Yotnda

Center for Cell and Gene Therapy, Baylor College of Medicine

Here we propose simple methods to test and evaluate the presence of reactive oxygen species in cells.

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

JoVE 2122 8/26/2010

Revital Halevy, Lazar Shtirberg, Michael Shklyar, Aharon Blank

Schulich Faculty of Chemistry, The Technion, Israel Institute of Technology

This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

JoVE 1305 6/09/2009

Christina Joselevitch, David Zenisek,

Cellular and Molecular Physiology, Yale University School of Medicine

In this video, we demonstrate how to label and visualize single synaptic vesicle exocytosis and trafficking in goldfish retinal bipolar cells using total internal reflectance fluorescence (TIRF) microscopy.

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

JoVE 2636 2/11/2011

J. Lowry Curley, Scott R. Jennings, Michael J. Moore

Biomedical Engineering, Tulane University

Simple techniques are described for the rapid production of microfabricated neural culture systems using a digital micromirror device for dynamic mask projection lithography on regular cell culture substrates. These culture systems may be more representative of natural biological architecture, and the techniques described could be adapted for numerous applications.

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

JoVE 3713 12/12/2011

Ling Liu¹, Steven R. Sansing¹, Iva S. Morse¹, Kathleen R. Pritchett-Corning²

¹Genetically Engineered Models and Services, Charles River , ²Research Models and Services, Charles River

Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.

Visualization of Vascular Ca²⁺ Signaling Triggered by Paracrine Derived ROS

JoVE 3511 12/21/2011

Karthik Mallilankaraman¹, Rajesh Kumar Gandhirajan¹, Brian J. Hawkins², Muniswamy Madesh¹

¹Department of Biochemistry, Temple University , ²Department of Anesthesiology and Pain Medicine, University of Washington

An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.

Plasma Lithography Surface Patterning for Creation of Cell Networks

JoVE 3115 6/14/2011

Michael Junkin¹, Siu Ling Leung¹, Yongliang Yang¹, Yi Lu¹, Justin Volmering¹, Pak Kin Wong^1,2

¹Aerospace and Mechanical Engineering, University of Arizona , ²Biomedical Engineering IDP and BIO5 Institute, University of Arizona

A versatile plasma lithography technique has been developed to generate stable surface patterns for guiding cellular attachment. This technique can be applied to create cell networks including those that mimic natural tissues and has been used for studying several, distinct cell types.