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Gastrula: The developmental stage that follows Blastula. It is characterized by the morphogenetic cell movements including invagination, ingression, and involution. Gastrulation begins with the formation of the Primitive streak, and ends with the formation of three Germ layers, the body plan of the mature organism.
 JoVE Biology

Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation

1Department of Biological Sciences, Smith College


JoVE 1422

Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.

 JoVE Biology

Primary Cell Cultures from Drosophila Gastrula Embryos

1Department of Genetics, Harvard Medical School, 2Howard Hughes Medical Institute


JoVE 2215

We provide a detailed protocol for preparing primary cells dissociated from Drosophila embryos. The ability to carry out the effective RNAi perturbation, together with other molecular, biochemical and cell imaging methods will allow a variety of questions to be addressed in Drosophila primary cells.

 JoVE Biology

Generating Chimeric Zebrafish Embryos by Transplantation

1HHMI and Division of Basic Sciences, Fred Hutchinson Cancer Research Center - FHCRC


JoVE 1394

A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

 JoVE Biology

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

1The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, 2Department of Developmental Biology, Washington University School of Medicine


JoVE 2010

This video protocol demonstrates a method for generating transgenic Xenopus laevis by introduction of transgenes into sperm nuclei followed by nuclear transplantation into unfertilized eggs.

 JoVE Biology

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

1Department of Biological Sciences, The George Washington University, 2Department of Anatomy and Regenerative Biology, The George Washington University


JoVE 4458

The fate of an individual embryonic cell can be influenced by inherited molecules and/or by signals from neighboring cells. Utilizing fate maps of the cleavage stage Xenopus embryo, single blastomeres can be identified for culture in isolation to assess the contributions of inherited molecules versus cell-cell interactions.

 JoVE Biology

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

1Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, 2Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics, University of Utah


JoVE 50038

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.

 JoVE Biology

Single Cell Fate Mapping in Zebrafish

1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 2Division of Hematology/Oncology, Cincinnati Children's Hospital Medical Center


JoVE 3172

A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.

 JoVE Biology

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

1Department of Development and Cell Biology, Department of Anatomy and Neurobiology, University of California, Irvine (UCI)


JoVE 200

This video demonstrates the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. Views of live cultures show neurite outgrowth and imaging of calcium levels using Fura-2.

 JoVE Neuroscience

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

1Biology Department, Skidmore College


JoVE 50703

Developmental processes such as proliferation, patterning, differentiation, and axon guidance can be readily modeled in the zebrafish spinal cord. In this article, we describe a mounting procedure for zebrafish embryos, which optimizes visualization of these events.

 JoVE Biology

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

1Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 2Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, 3Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University


JoVE 2686

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

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