JoVE Immunology and Infection
Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
Centre for Immunity, Infection and Evolution, University of Edinburgh
An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines.
JoVE Bioengineering
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
1Center for Biophotonics Science and Technology, University of California, Davis, 2Department of Pathology and Laboratory Medicine, University of California, Davis
We discuss the construction and operation of a complex nonlinear optical system that uses ultrafast all-optical switching to isolate Raman from fluorescence signals. Using this system we are able to successfully separate Raman and fluorescence signals utilizing pulse energies and average powers that remain biologically safe.
JoVE General
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
We describe the prototypical method for producing microscope coverslips coated with fluorescent gelatin for visualizing invadopodia-mediated matrix degradation. Computational techniques using available software are presented for quantifying the resultant levels of matrix proteolysis by single cells within a mixed population and for multicellular groups encompassing entire microscopic fields.
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