JoVE 7th Issue: Bioengineering and Immunology

JoVE 274 8/30/2007

JoVE

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

JoVE 3738 4/16/2012

Gavin I. Ellis, Mary Catherine Reneer, Alejandra Catalina Vélez-Ortega, Andrea McCool, Francesc Martí

Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

Measuring the 50% Haemolytic Complement (CH₅₀) Activity of Serum

JoVE 1923 3/29/2010

Maurizio Costabile

School of Pharmacy and Medical Sciences, University of South Australia

The classical pathway is activated by antibody and culminates in target cell lysis. The CH₅₀ assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH₅₀ of a serum sample, the calculations and interpreting of results.

June 2011: This Month in JoVE

JoVE 3549 6/01/2011

Aaron Kolski-Andreaco

JoVE Issue 8: Microfluidics, cell patterning and more.

JoVE 324 10/01/2007

JoVE Editors

JoVE

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

JoVE 556 12/10/2007

Archana Khurana, Mitchell Kronenberg

Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology

A Method to prepare Insect cells and infect them with baculovirus for the the purpose of production of recombinant mCD1d proteinand generating mCD1d tetramers.

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation

JoVE 635 12/31/2007

Mitchell Kronenberg

Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology

Natural Killer T cells (NKT) are critical determinants of the immune response to cancer, regulation of autioimmunity, clearance of infection, and the development of artheriosclerotic plaques. In this interview, Mitch Kronenberg discusses his laboratory's efforts to understand the mechanism through which NKT cells are activated by glycolipid antigens.

Bio-Plex® Multi-Plex Immunoassay Overview and Testimonials - ADVERTISEMENT

JoVE 3537 6/15/2011

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

JoVE 2259 10/12/2010

Benjamin J. C. Quah, Christopher R. Parish

Department of Immunology, John Curtin School of Medical Research, Australian National University

CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.

Isolation of Brain-infiltrating Leukocytes

JoVE 2747 6/13/2011

Reghann G. LaFrance-Corey*, Charles L. Howe*

Department of Neurology, Mayo Clinic College of Medicine

A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.