Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

JoVE 1265 10/06/2009

Bethanie Morrison, Mary Lou Cutler

Department of Pathology, F. Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD

HC11 lactogenic differentiation can be characterized by the formation of domed structures referred to as mammospheres. The structures can be enumerated by phase contrast microscopy to aid in quantifying lactogenic differentiation.