Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

JoVE 1690 2/16/2010

Matias Pasquali, Frédéric Giraud, Jean Paul Lasserre, Sebastien Planchon, Lucien Hoffmann, Torsten Bohn, Jenny Renaut

Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox), Centre de Recherche Public-Gabriel Lippmann

Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.