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 JoVE Biology

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

1Research Animal Diagnostic Services (RADS), Charles River


JoVE 3715

Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.

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