High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

JoVE 3746 5/10/2012

Sucharit Katyal, Clint A. Greene, David Ress

Psychology & Neurobiology, Imaging Research Center & Center for Perceptual Systems, The University of Texas at Austin

This article describes techniques to perform high-resolution functional magnetic resonance imaging with 1.2 mm sampling in human midbrain and subcortical structures using a 3T scanner. Use of these techniques to resolve topographic maps of visual stimulation in the human superior colliculus (SC) is given as an example.

Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy

JoVE 3007 8/08/2011

Ian Sharp¹, James Patton¹, Molly Listenberger², Emily Case²

¹Department of Bioengineering, University of Illinois at Chicago and Rehabilitation Institute of Chicago, ²Sensory Motor Performance Program, Rehabilitation Institute of Chicago

Recently, a vast amount of prospects have come available for human-robot interactive systems. In this paper we outline the integration of a new robotic device with open source software that can rapidly make possible a library of interactive functionality. We then outline a clinical application for a neurorehabilitation application.

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

JoVE 1154 5/13/2009

Dee Brink, Mary Gilbert, Vanessa Auld

Department of Zoology, University of British Columbia - UBC

We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans.

Investigating Social Cognition in Infants and Adults Using Dense Array Electroencephalography (_dEEG)

JoVE 2759 6/27/2011

Adekemi J. Akano, David W. Haley, Joanna Dudek

Department of Psychology, University Toronto Scarborough

Dense array electroencephalography is being used increasingly to study social cognitive functions in infants and adults. Here we present an established methodology that represents a significant improvement on conventional methodologies for studying EEG in infants and adults.

Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions

JoVE 1133 3/16/2009

Nicholas J. Hornstein, Stefan R. Pulver, Leslie C. Griffith

Department of Biology, Brandeis

This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.

Visually Mediated Odor Tracking During Flight in Drosophila

JoVE 1110 1/26/2009

Mark A. Frye, Brian J. Duistermars

Department of Physiological Science, University of California, Los Angeles

Here we describe how to optimize the acquired video image for an olfactory magnetic-tether (OMT) apparatus. We also describe two sample experimental protocols for studying visuo-olfactory fusion.

A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila

JoVE 1063 11/21/2008

Brian J. Duistermars , Mark A. Frye

Department of Physiological Science, University of California, Los Angeles

Here we describe how to tether a fly in an olfactory magnetic-tether (OMT) apparatus. We describe how to align the rare-earth magnets and odor ports, and how to set mass flow rates for both the stimulus delivery and vacuum suction to achieve optimal odor tracking.

Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze

JoVE 2155 10/13/2010

Prasad R. Shirvalkar, Mathew L. Shapiro

Department of Neuroscience, Friedman Brain Institute, Mount Sinai School of Medicine

We present a low-cost method to design and construct a light headstage pre-amplifier system with simultaneous neural recording and stimulation capability. This device can be waterproofed for use in swimming animals.

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior

JoVE 2273 12/28/2010

Umer Najib¹, Jared C. Horvath¹, Juha Silvanto², Alvaro Pascual-Leone¹

¹Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center, ²Brain Research Unit, Low Temperature Laboratory and Advanced magnetic Imaging Center, Aalto University School of Science and Technology

In this article, we examine the effects of visually relevant state dependency on TMS induced motive phosphenic presentations.

December 2011: This Month in JoVE

JoVE 4114 12/01/2011

Aaron Kolski-Andreaco