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 JoVE Biology

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University


JoVE 3583

13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.

 JoVE Biology

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

1Molekulare Pflanzenphysiologie, Max-Planck-Institut


JoVE 3487

Combination of genomics, co-expression gene analysis and the identification of target compounds via metabolism give gene functional annotation.

 JoVE Neuroscience

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

1MSTP, Neuroscience Program, Washington University in St. Louis, 2Washington University Pain Center, Department of Anesthesiology, Washington University in St. Louis


JoVE 52640

The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.

 JoVE Behavior

Disrupting Reconsolidation of Fear Memory in Humans by a Noradrenergic β-Blocker

1Department of Clinical Psychology, University of Amsterdam


JoVE 52151

Disrupting reconsolidation is a promising approach to dampen the behavioral expression of fear memory in patients with anxiety disorders or posttraumatic stress disorder. In a series of human fear conditioning studies we showed that disrupting reconsolidation by the noradrenergic β-blocker propranolol is very effective in erasing conditioned fear responding.

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