1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University
13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.
Published January 26, 2012. Keywords: Molecular Biology, GC-MS, novel pathway, metabolism, labeling, phototrophic microorganism
1Molekulare Pflanzenphysiologie, Max-Planck-Institut
Combination of genomics, co-expression gene analysis and the identification of target compounds via metabolism give gene functional annotation.
Published June 17, 2012. Keywords: Plant Biology, Genetics, Bioinformatics, Metabolomics, Plant metabolism, Transcriptome analysis, Functional annotation, Computational biology, Plant biology, Theoretical biology, Spectroscopy and structural analysis
1MSTP, Neuroscience Program, Washington University in St. Louis, 2Washington University Pain Center, Department of Anesthesiology, Washington University in St. Louis
The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.
Published March 17, 2015. Keywords: Neuroscience, Neuroscience, mouse, behavior, reflex, cold, thermosensation, adaptation, acetone, cold plate
1Department of Clinical Psychology, University of Amsterdam
Disrupting reconsolidation is a promising approach to dampen the behavioral expression of fear memory in patients with anxiety disorders or posttraumatic stress disorder. In a series of human fear conditioning studies we showed that disrupting reconsolidation by the noradrenergic β-blocker propranolol is very effective in erasing conditioned fear responding.
Published December 18, 2014. Keywords: Behavior, Fear memory, reconsolidation, noradrenergic β-blocker, human fear conditioning, startle potentiation, translational research.