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 JoVE Chemistry

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

1Complex Carbohydrate Research Center, University of Georgia, 2Department of Biochemistry and Molecular Biology, University of Georgia, 3Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University


JoVE 51840

In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.

 JoVE Biology

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

1New England Biolabs


JoVE 3749

Using specific glycosidases to remove sugars from glycoproteins followed by SDS-PAGE is a valuable method to detect glycan modifications on protein samples and is a good choice for initial glycobiology studies. Changes following deglycosylation can be detected as shifts in gel mobility or by staining with glycan sensitive reagents.

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