Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

JoVE 3562 1/17/2012

Qing-Yun Tian¹, Yun-Peng Zhao¹, Chuan-ju Liu^1,2

¹Department of Orthopaedic Surgery, NYU Hospital for Joint Diseases, ²Department of Cell Biology, New York University School of Medicine

We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.