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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET


JoVE 3410 1/27/2012

Department of Cell and Molecular Biology, Karolinska Institutet

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

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