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Potassium Ionophores: Chemical agents that increase the permeability of Cell membranes to Potassium ions.
 JoVE Immunology and Infection

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

1Department of Microbiology and Immunology, University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York


JoVE 51008

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 JoVE Chemistry

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST, Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST, Center for Nanotechnology Innovation


JoVE 50545

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 Science Education: Essentials of Environmental Science

Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium

JoVE Science Education

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University

In this experiment, three soil macronutrients are chemically extracted, combined with color-based reagents, then analyzed using color to determine the nutrient concentration present in the soil sample.

Nitrogen, phosphorus, and potassium are the main components of soil fertilizer. These methods isolate each nutrient from the soil into a solution that can be analyzed using turbidity and color to determine the concentration of nutrients present in the soil sample. Knowing present concentration informs environmental scientists of a nutrient deficiency or surplus in soils used to support plant production, and also provides general insight into basic biogeochemical cycles of an ecosystem.

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 JoVE Biology

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

1Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, 2Departamento de Biologia, Centro de Biologia Molecular e Ambiental, Universidade do Minho, 3Department of Neurology and Center for Neuroscience, University of California, Davis Imaging of Dementia and Aging Laboratory, 4Department of Ophthalmology, Institute for Regenerative Cures, University of California, Davis


JoVE 52782

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 JoVE Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota


JoVE 50762

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 JoVE Biology

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie, Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind, University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke, National Institute of Health


JoVE 52281

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 JoVE Biology

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

1Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Hanover, NH, 2Environmental Processes Branch, Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS, 3Terrestrial and Cryospheric Scienes Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Hanover, NH, 4Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Fairbanks, AK


JoVE 54091

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 Science Education: Essentials of General Chemistry

Le Châtelier's Principle

JoVE Science Education

Source: Laboratory of Dr. Lynne O'Connell — Boston College

When the conditions of a system at equilibrium are altered, the system responds in such a way as to maintain the equilibrium. In 1888, Henri-Lewis Le Châtelier described this phenomenon in a principle that states, "When a change in temperature, pressure, or concentration disturbs a system in chemical equilibrium, the change will be counteracted by an alteration in the equilibrium composition." This experiment demonstrates Le Châtelier's principle at work in a reversible reaction between iron(III) ion and thiocyanate ion, which produces iron(III) thiocyante ion: Fe3+(aq) + SCN- (aq) FeSCN2+ (aq) The concentration of one of the ions is altered either by directly adding a quantity of one ion to the solution or by selectively removing an ion from the solution through formation of an insoluble salt. Observations of color changes indicate whether the equilibrium has shifted to favor formation of the products or the reactants. In addition, the effect of a temperature change on the solution at equilibrium can be obs

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 Science Education: Essentials of General Chemistry

Introduction to Titration

JoVE Science Education

Source: Laboratory of Dr. Yee Nee Tan — Agency for Science, Technology, and Research

Titration is a common technique used to quantitatively determine the unknown concentration of an identified analyte.1-4 It is also called volumetric analysis, as the measurement of volumes is critical in titration. There are many types of titrations based on the types of reactions they exploit. The most common types are acid-base titrations and redox titrations.5-11 In a typical titration process, a standard solution of titrant in a burette is gradually applied to react with an analyte with an unknown concentration in an Erlenmeyer flask. For acid-base titration, a pH indicator is usually added in the analyte solution to indicate the endpoint of titration.12 Instead of adding pH indicators, pH can also be monitored using a pH meter during a titration process and the endpoint is determined graphically from a pH titration curve. The volume of titrant recorded at the endpoint can be used to calculate the concentration of the analyte based on the reaction stoichiometry. For the acid-base titration presented in this video, the titrant is a standardized sodium hydroxide solution and the analyte is domestic vinegar. Vinegar is an acidic liquid that

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 JoVE Genetics

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

1School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, 2Victorian AgriBiosciences Centre, La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology, University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria


JoVE 54553

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