Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

JoVE 3518 4/23/2012

Nazim El-Andaloussi*^1,2, Barbara Leuchs*¹, Serena Bonifati^1,2, Jean Rommelaere^1,2, Antonio Marchini^1,2

¹Tumour Virology Division F010, German Cancer Research Center (DKFZ), ²Inserm Unit 701, German Cancer Research Center (DKFZ)

Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).