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  JoVE Developmental Biology

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 JoVE Developmental Biology

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery, Swiss Paraplegic Centre, 3Department of Neurosurgery, Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology, Vetsuisse Faculty, University of Zurich


JoVE 52694

Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

 JoVE Biology

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

1Department of Genetics, University of Georgia


JoVE 2797

This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

 JoVE Biology

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate

1Sigma Aldrich


JoVE 899

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.

 JoVE Biology

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

1Biochemistry and Molecular Biology, Colorado State University


JoVE 50354

A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

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