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 JoVE Developmental Biology

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery, Swiss Paraplegic Centre, 3Department of Neurosurgery, Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology, Vetsuisse Faculty, University of Zurich


JoVE 52694

Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

 JoVE Biology

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

1Department of Genetics, University of Georgia


JoVE 2797

This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

 JoVE Biology

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate

1Sigma Aldrich


JoVE 899

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.

 JoVE Biology

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

1Biochemistry and Molecular Biology, Colorado State University


JoVE 50354

A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

 JoVE Application Notes

Sigma公司的以酪蛋白为底物泛蛋白酶活性检测方法 - ADVERTISEMENT

1Sigma Aldrich


JoVE 910

概要 Sigma公司发展和标准化蛋白酶活性检测技术。在这个泛蛋白酶活动检测中以酪蛋白为底物。这检测法的原理是当蛋白酶消化酪蛋白时,酪氨酸从其他氨基酸和肽段中释放出来。游离的酪氨酸与福林酚试剂反应生产蓝色基,并可用分光光度计660纳米波长测量吸光度。蛋白酶活动产生的吸光度值与标准曲线相比较,这曲线是由已知量的酪氨酸与福林酚试剂反应而引起吸光度变化与酪氨酸微摩尔量关౿

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