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 JoVE Biology

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

1Department of Biology, Miami University


JoVE 52059

RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.

 JoVE Biology

Preparing Individual Drosophila Egg Chambers for Live Imaging

1Department of Biochemistry, University of Oxford


JoVE 3679

The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.

 JoVE Biology

Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish

1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah


JoVE 2689

Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.

 JoVE Neuroscience

Neural Explant Cultures from Xenopus laevis

1Department of Cell Biology, Harvard Medical School


JoVE 4232

Culturing neural explants from dissected Xenopus laevis embryos that express fluorescent fusion proteins allows for imaging of growth cone cytoskeletal dynamics.

 JoVE Biology

Immunostaining of Dissected Zebrafish Embryonic Heart

1Division of Cardiovascular Diseases, Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine


JoVE 3510

A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.

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