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Role Playing: The adopting or performing the role of another significant individual in order to gain insight into the behavior of that person.
 JoVE Medicine

A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice

1Johns Hopkins University School of Medicine, 2Burn and Complex Wound Center, 3Section of Plastic and Reconstructive Surgery, University of Chicago Medical Center, 4Division of Plastic, Reconstructive, and Maxillofacial Surgery, R Adams Cowley Shock Trauma Center, 5Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, 6Vascularized Composite Allotransplantation (VCA) Lab, Johns Hopkins University School of Medicine


JoVE 53442

 JoVE Chemistry

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

1Laboratory for Molecular Cancer Biology, Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, 2Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University


JoVE 53563

 Science Education: Essentials of Experimental Psychology

From Theory to Design: The Role of Creativity in Designing Experiments

JoVE Science Education

Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University

Research studies come into being when a researcher speculates about human thought, emotions, or behavior, and has a theory that offers a potential explanation. Often the researcher’s theory is firmly situated in everyday common experiences that may not naturally lend themselves to direct empirical study. For example, researchers speculated that perception of a person on Facebook is influenced by the appearances and comments of the person’s Facebook friends.1 It is difficult to test this theory using real-life Facebook profiles. Instead, researchers must use their creativity to design a study—in this case, using fake profiles that look highly realistic—to test their theory.  This video demonstrates how researchers test a central tenet of a popular social psychology theory. Specifically, this video shows a test of whether engaging in a self-expanding activity leads a person to feel a greater sense of self-efficacy.2 Psychological studies often use higher sample sizes than studies in other sciences. A large number

 JoVE Developmental Biology

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine


JoVE 52544

 Science Education: Essentials of Developmental Psychology

Executive Function and the Dimensional Change Card Sort Task

JoVE Science Education

Source: Laboratories of Nicholaus Noles and Judith Danovitch—University of Louisville

Infants are born with amazing cognitive resources at their disposal, but they don’t know how to use them effectively. In order to harness the power of their brains, humans must develop high-level cognitive processes that manage basic brain functions. These processes make up what psychologists refer to as executive function. Executive function is a key factor in many self-regulatory behaviors, including forming plans to solve problems, negotiating between desires and actions, and directing attention. For example, a child must use several executive processes to stop playing with toys and start cleaning their room. These processes include inhibition (to stop what they’re doing), planning (to determine what actions need to be performed to clean the room), and attentional control (to stay on task until the cleaning is done). A breakdown of executive function during any of these steps would lead to the room remaining dirty. Developing executive function is one of the key challenges faced by children as they mature. Some elements of executive function can only be mastered with practice, and brain areas linked to executive function, specifically the prefrontal cortex, develop slowly throughout

 Science Education: Essentials of Neuropsychology

Measuring Grey Matter Differences with Voxel-based Morphometry: The Musical Brain

JoVE Science Education

Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California

Experience shapes the brain. It is well understood that our brains are different as a result of learning. While many experience-related changes manifest themselves at the microscopic level, for example by neurochemical adjustments in the behavior of individual neurons, we may also examine anatomical changes to the structure of the brain at a macroscopic level. One famous example of this kind of change comes from the case of the London taxi drivers, who along with learning the complex routes of the city show larger volume in the hippocampus, a brain structure known to play a role in navigational memory.1 Many traditional methods of examining brain anatomy require painstaking tracing of anatomical regions of interest in order to measure their size. However, using modern neuroimaging techniques, we can now compare the anatomy of the brains across groups of people using automated algorithms. While these techniques do not avail themselves of the sophisticated knowledge that human neuroanatomists may bring to the task, they are quick, and sensitive to very small differences in anatomy. In a structural magnetic resonance image of the brain, the intensity of each volumetric pixel, or voxel, relat

 JoVE In-Press

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

1Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

Video Coming Soon

JoVE 55095

 JoVE Environment

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

1Center for Cancer Pharmacology, University of Pennsylvania, 2Center for Excellence in Environmental Toxicology, University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, 5A.J. Drexel Autism Institute, Drexel University


JoVE 53941

 JoVE Cancer Research

Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones

1Department of Surgery, The University of Chicago, 2Department of Radiation and Cellular Oncology and Ludwig Center for Metastasis Research, The University of Chicago


JoVE 54657

 JoVE Medicine

Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue

1The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 2Department of Oncology, The Johns Hopkins University School of Medicine, 3Department of Dermatology, The Johns Hopkins University School of Medicine, 4Department of Surgery, Johns Hopkins University School of Medicine, 5The Sol Goldman Pancreatic Cancer Center, The Johns Hopkins University School of Medicine, 6Yale Cancer Center, Yale School of Medicine, 7The Skip Viragh Center for Pancreatic Cancer, The Johns Hopkins University School of Medicine, 8Department of Pathology, The Johns Hopkins University School of Medicine


JoVE 4059

 JoVE Medicine

A Multimodal Imaging- and Stimulation-based Method of Evaluating Connectivity-related Brain Excitability in Patients with Epilepsy

1Department of Neurology, Harvard Medical School, 2Department of Neurology, Beth Israel Deaconess Medical Center, 3Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center, 4Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, 5Department of Neurology, Massachusetts General Hospital


JoVE 53727

 JoVE Immunology and Infection

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research


JoVE 4322

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