Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

JoVE 3894 2/21/2012

Toni G. Patton¹, Gabrielle Dietrich², Kevin Brandt¹, Marc C. Dolan², Joseph Piesman², Robert D. Gilmore Jr.¹

¹Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, ²Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention

The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults.

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

JoVE 228 7/04/2007

Judy Coleman¹, Jennifer Juhn¹, Anthony A. James²

¹Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI), ²Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics, University of California, Irvine (UCI)

The mosquito midgut and salivary glands are key entry and exit points for vector pathogens like Plasmodium falciparum and the dengue virus. This video demonstrates the dissection techniques for removing the midgut and salivary glands from Aedes aegypti mosquitoes.

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

JoVE 3074 5/14/2011

Yusuke Kuriki¹, Younan Liu¹, Dengsheng Xia¹, Eva M. Gjerde¹, Saeed Khalili¹, Brennan Mui¹, Changyu Zheng², Simon D. Tran¹

¹Faculty of Dentistry, McGill University , ²National Institutes of Health, Bethesda, MD, USA

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.

Isolation of Mouse Salivary Gland Stem Cells

JoVE 2484 2/08/2011

Sarah Pringle^1,2, Lalitha S. Y. Nanduri¹, van der Zwaag Marianne ¹, van Os Ronald^1,2, Rob P. Coppes^1,2

¹Department of Cell Biology, University Medical Center Groningen, University of Groningen, ²Department of Radiation Oncology, University Medical Center Groningen, University of Groningen

An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

JoVE 1510 8/20/2009

Claudio Vinegoni¹, Daniel Razansky², Chrysoula Pitsouli³, Norbert Perrimon³, Vasilis Ntziachristos², Ralph Weissleder¹

¹Center for Systems Biology, Massachusetts General Hospital, ²Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, ³Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

JoVE 1748 2/09/2010

Weili Cai, Ye Jin, Jack Girton, Jorgen Johansen, Kristen M. Johansen

Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University

This video protocol illustrates the squash technique used in the Johansen laboratory to prepare Drosophila polytene chromosomes for antibody labeling.

February 2012: This Month in JoVE

JoVE 4258 2/01/2012

Aaron Kolski-Andreaco

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

JoVE 700 5/14/2008

Cecilia Tamborindeguy¹, Stewart Gray¹, Georg Jander²

¹Plant Pathology, Cornell University, ²Boyce Thompson Institute for Plant Research, Cornell University

Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

JoVE 1155 7/09/2009

Patrick Cafferty, Xiaojun Xie, Kristen Browne, Vanessa J. Auld

Department of Zoology, University of British Columbia - UBC

Here we describe a protocol to examine the migration of glial cells into the developing Drosophila eye using live microscopic analysis paired with GFP tagged glial cells.

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

JoVE 3347 5/07/2012

Chiyedza Small*^1,2, Indira Paddibhatla*^1,2, Roma Rajwani¹, Shubha Govind^1,2

¹Biology Department, The City College of New York, CUNY, ²The Graduate Center, The City University of New York

Parasitoid (parasitic) wasps constitute a major class of natural enemies of many insects including Drosophila melanogaster. We will introduce the techniques to propagate these parasites in Drosophila spp. and demonstrate how to analyze their effects on immune tissues of Drosophila larvae.